Appearance of electron spin resonance signals in the interaction of dithiocarbamate-Fe(II) with nitrogen dioxide and nitrite

被引：23

作者：

Hiramoto, K ^{[1
]}

Tomiyama, S ^{[1
]}

Kikugawa, K ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Pharm, Tokyo 19203, Japan

来源：

FREE RADICAL RESEARCH | 1997年 / 27卷 / 05期

关键词：

dithiocarbamate-Fe(II); dithiocarbamate-Fe(II)-NO spin adduct; nitrogen monooxide; nitrogen dioxide; nitrite;

D O I：

10.3109/10715769709065790

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Whether dithiocarbamate-Fe(II) complexes used in the electron spin resonance (ESR) studies are specific for the detection of NO was investigated. It was found that the typical ESR signals of dithiocarbamate-Fe(II)-NO spin adducts appeared in the interaction of N-methyl-D-glucaminedithiocarbamate (MGD)-, bis(hydroxyethyl) dithiocarbamate (HED)- and diethyldithiocarbamate (DED)-Fe(II) complexes with the NO-derived nitrogen oxides, nitrogen dioxide (NO2) and nitrite ion (NO2-) in a prolonged incubation. Under the conditions of prolonged incubation, appearance of the ESR signals of the dithiocarbamate-Fe(II)-NO spin adducts may indicate the presence of not only NO but NO2 and NO2-.

引用

页码：505 / 509

页数：5

共 17 条

[1]

ADAMS LB, 1990, J IMMUNOL, V144, P2725

[2]

BECKMAN JS, 1994, METHOD ENZYMOL, V233, P229

[3]

CZAFIRIOU OC, 1980, ANAL CHEM, V52, P162

[4] Colorimetric determination of dl-x-tocopherol (vitamin E) [J].

Emmerie, A ;

Engel, C .

NATURE, 1938, 142 :873-873

[5] KINETICS OF NITRIC-OXIDE AUTOXIDATION IN AQUEOUS-SOLUTION IN THE ABSENCE AND PRESENCE OF VARIOUS REDUCTANTS - THE NATURE OF THE OXIDIZING INTERMEDIATES [J].

GOLDSTEIN, S ;

CZAPSKI, G .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (49) :12078-12084

[6] IN-VIVO SPIN-TRAPPING OF NITRIC-OXIDE IN MICE [J].

KOMAROV, A ;

MATTSON, D ;

JONES, MM ;

SINGH, PK ;

LAI, CS .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 195 (03) :1191-1198

[7] PEROXYNITRITE, A CLOAKED OXIDANT FORMED BY NITRIC-OXIDE AND SUPEROXIDE [J].

KOPPENOL, WH ;

MORENO, JJ ;

PRYOR, WA ;

ISCHIROPOULOS, H ;

BECKMAN, JS .

CHEMICAL RESEARCH IN TOXICOLOGY, 1992, 5 (06) :834-842

[8] SPIN-TRAPPING OF NITRIC-OXIDE PRODUCED IN-VIVO IN SEPTIC-SHOCK MICE [J].

LAI, CS ;

KOMAROV, AM .

FEBS LETTERS, 1994, 345 (2-3) :120-124

[9]

LIEW FY, 1990, J IMMUNOL, V144, P4794

[10] Stimulation and inhibition of nitric oxide production in macrophages and neural cells as observed by spin trapping [J].

Norby, SW ;

Weyhenmeyer, JA ;

Clarkson, RB .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (1-2) :1-9

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