Separation of Cimicifuga racemosa triterpene glycosides by reversed phase high performance liquid chromatography and evaporative light scattering detection

被引：27

作者：

Ganzera, M

Bedir, E

Khan, IA ^{[1
]}

机构：

[1] Univ Mississippi, Sch Pharm, Natl Ctr Nat Prod Res, Res Inst Pharmaceut Sci, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA

[3] Hacettepe Univ, Fac Pharm, Dept Pharmacognosy, TR-06100 Ankara, Turkey

来源：

CHROMATOGRAPHIA | 2000年 / 52卷 / 5-6期

关键词：

column liquid chromatography; evaporative light scattering detection; Cimicifuga racemosa; black cohosh; triterpene glycosides;

D O I：

10.1007/BF02491021

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The main triterpene glycosides of Cimicifuga racemosa were separated by reversed phase HPLC, using a C-18 column, Evaporative Light Scattering (ELS) detection and a gradient system consisting of water, acetonitrile and reagent alcohol. Within 35 min three main glycosides could be separated and quantified in the methanolic root extract, with detection limits of 10.5, 15.6 and 31.6 mu g . mL(-1) respectively The method was successfully used to analyze different Cimicifuga racemosa market products, as well as to distinguish between other Cimicifuga samples from China.

引用

页码：301 / 304

页数：4

共 9 条

[1]

Bedir E, 2000, CHEM PHARM BULL, V48, P425

[2] EFFECTS OF EXTRACTS FROM CIMICIFUGA-RACEMOSA ON GONADOTROPIN-RELEASE IN MENOPAUSAL WOMEN AND OVARIECTOMIZED RATS [J].