Effects of berberine on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adduct formation in human leukemia cells

被引：21

作者：

Chung, JG ^{[1
]}

Chen, GW ^{[1
]}

Hung, CF ^{[1
]}

Lee, JH ^{[1
]}

Ho, CC ^{[1
]}

Ho, HC ^{[1
]}

Chang, HL ^{[1
]}

Lin, WC ^{[1
]}

Lin, JG ^{[1
]}

机构：

[1] China Med Coll, Dept Microbiol, Taichung 400, Taiwan

来源：

AMERICAN JOURNAL OF CHINESE MEDICINE | 2000年 / 28卷 / 02期

关键词：

D O I：

10.1142/S0192415X00000271

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

Berberine is an alkaloid occurring in the plant genera Berberis and Coptis. Although berberine had been demonstrated to have antineoplastic function by inhibiting DNA-synthesis in activated lymphocytes, there is no available information to address berberine affects on human leukemia cell N-acetyltransferase (NAT) activity and 2-aminofluorene (AF)-DNA adduct formation. Thus, berberine was tested for inhibition of arylamine NAT activity and AF-DNA adduct formation in human leukemia cells. The NAT activity was measured by a high performance liquid chromatography assaying for the amounts of N-acetyl-2-aminofluorene (AAF) and N-acetyl-p-aminobenzoic acid (N-Ac-PABA) and the remaining AF and p-aminobenzoic acid (PABA). The NAT activity and AF-DNA adduct formation in human leukemia cells were inhibited by berberine in a dose-dependent manner, i.e. the higher the concentration of berberine, the higher the inhibition of NAT activity and AF-DNA adduct. The data also indicate that berberine decreased the apparent values of Km and Vmax from human leukemia cells in both cytosol acid intact cells.

引用

页码：227 / 238

页数：12

共 30 条

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