Nuclear pore complex structure and dynamics revealed by cryoelectron tomography

被引：388

作者：

Beck, M ^{[1
]}

Förster, F ^{[1
]}

Ecke, M ^{[1
]}

Plitzko, JM ^{[1
]}

Melchior, F ^{[1
]}

Gerisch, G ^{[1
]}

Baumeister, W ^{[1
]}

Medalia, O ^{[1
]}

机构：

[1] Max Planck Inst Biochem, D-82152 Martinsried, Germany

来源：

SCIENCE | 2004年 / 306卷 / 5700期

关键词：

D O I：

10.1126/science.1104808

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nuclear pore complexes (NPCs) are gateways for nucleocytoplasmic exchange. To analyze their structure in a close-to-life state, we studied transport-active, intact nuclei from Dictyostelium discoideum by means of cryoelectron tomography. Subvolumes of the tomograms containing individual NPCs were extracted in silico and subjected to three-dimensional classification and averaging, whereby distinct structural states were observed. The central plug/transporter (CP/T) was variable in volume and could occupy different positions, along the nucleocytoplasmic axis, which supports the notion that it essentially represents cargo in transit. Changes in the position of the CP/T were accompanied by structural rearrangements in the NPC scaffold.

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页码：1387 / 1390

页数：4

相关论文

共 16 条

[1] ARCHITECTURE OF THE XENOPUS NUCLEAR-PORE COMPLEX REVEALED BY 3-DIMENSIONAL CRYOELECTRON MICROSCOPY [J].

AKEY, CW ;

RADERMACHER, M .

JOURNAL OF CELL BIOLOGY, 1993, 122 (01) :1-19

[2] Electron tomography: towards visualizing the molecular organization of the cytoplasm [J].

Baumeister, W .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2002, 12 (05) :679-684

[3] The nuclear pore complex: Nucleocytoplasmic transport and beyond [J].

Fahrenkrog, B ;

Aebi, U .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (10) :757-766

[4] The nuclear pore complex and lamina: Three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy [J].

Goldberg, MW ;

Allen, TD .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 257 (04) :848-865

[5] Prospects of electron cryotomography to visualize macromolecular complexes inside cellular compartments:: implications of crowding [J].

Grünewald, K ;

Medalia, O ;

Gross, A ;

Steven, AC ;

Baumeister, W .

BIOPHYSICAL CHEMISTRY, 2003, 100 (1-3) :577-591

[6] Yeast nuclear pore complexes have a cytoplasmic ring and internal filaments [J].

Kiseleva, E ;

Allen, TD ;

Rutherford, S ;

Bucci, M ;

Wente, SR ;

Goldberg, MW .

JOURNAL OF STRUCTURAL BIOLOGY, 2004, 145 (03) :272-288

[7]

Kiseleva E, 1998, J CELL SCI, V111, P223

[8] Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography [J].

Medalia, O ;

Weber, I ;

Frangakis, AS ;

Nicastro, D ;

Gerisch, G ;

Baumeister, W .

SCIENCE, 2002, 298 (5596) :1209-1213

[9] INHIBITION OF NUCLEAR-PROTEIN IMPORT BY NONHYDROLYZABLE ANALOGS OF GTP AND IDENTIFICATION OF THE SMALL GTPASE RAN/TC4 AS AN ESSENTIAL TRANSPORT FACTOR [J].

MELCHIOR, F ;

PASCHAL, B ;

EVANS, J ;

GERACE, L .

JOURNAL OF CELL BIOLOGY, 1993, 123 (06) :1649-1659

[10] Sequential binding of import ligands to distinct nucleopore regions during their nuclear import [J].

Pante, N ;

Aebi, U .

SCIENCE, 1996, 273 (5282) :1729-1732