Extracellular Ca2+ sensing contributes to excess Ca2+ accumulation and vacuolar fragmentation in a pmr1Δ mutant of S-cerevisiae

被引:49
作者
Kellermayer, R
Aiello, DP
Miseta, A
Bedwell, DM [1 ]
机构
[1] Univ Alabama Birmingham, Dept Microbiol, Birmingham, AL 35294 USA
[2] Univ Pecs, Sch Med, Dept Clin Chem, H-7624 Pecs, Hungary
关键词
yeast; Pmr1P; Ca2+ homeostasis; vacuole;
D O I
10.1242/jcs.00372
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Previous studies have suggested that yeast strains lacking the Ca2+-ATPase Pmr1p are unable to maintain an adequate level of Ca2+ within the Golgi apparatus. It is thought that this compartmental store depletion induces a signal that causes an increased rate of Ca2+ uptake and accumulation in a manner similar to the capacitative Ca2+ entry (CCE) response in non-excitable mammalian cells. To explore this model further, we examined cellular Ca2+ uptake and accumulation in a pmrlDelta strain grown in the presence of a reduced level of divalent cations. We found that the level of Ca2+ uptake and accumulation in a pmr1Delta strain increased as the concentration of divalent cations in the growth medium decreased. These results are inconsistent with a model in which cellular Ca2+ uptake and accumulation are determined solely by the depletion of Ca2+ in an intracellular compartment. Instead, our results suggest that a second regulatory mechanism couples cellular Ca2+ uptake to the availability of Ca2+ in the extracellular environment. Furthermore, we found that various conditions that increase the level of cytosolic Ca2+ correlate with vacuolar fragmentation in wild-type (WT), pmr1Delta and pmr1Delta/pmc1Delta yeast strains. This suggests that vacuolar fragmentation might function as a normal physiological response to Ca2+ stress that increases the vacuolar surface/volume ratio, thereby maximizing the sequestration of this important signaling molecule.
引用
收藏
页码:1637 / 1646
页数:10
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