The Drosophila immune system detects bacteria through specific peptidoglycan recognition

被引:477
作者
Leulier, F
Parquet, C
Pili-Floury, S
Ryu, JH
Caroff, M
Lee, WJ
Mengin-Lecreulx, D
Lemaitre, B [1 ]
机构
[1] CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France
[2] Univ Paris 11, CNRS, UMR 8619, IBBMC, F-91405 Orsay, France
[3] Ewha Womans Univ, Div Mol Life Sci, Mol & Cellular Biol Lab, Seoul 120750, South Korea
关键词
D O I
10.1038/ni922
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The Drosophila immune system discriminates between different classes of infectious microbes and responds with pathogen-specific defense reactions through selective activation of the Toll and the immune deficiency (Imd) signaling pathways. The Toll pathway mediates most defenses against Gram-positive bacteria and fungi, whereas the Imd pathway is required to resist infection by Gram-negative bacteria. The bacterial components recognized by these pathways remain to be defined. Here we report that Gram-negative diaminopimelic acid-type peptidoglycan is the most potent inducer of the Imd pathway and that the Toll pathway is predominantly activated by Gram-positive lysine-type peptidoglycan. Thus, the ability of Drosophila to discriminate between Gram-positive and Gram-negative bacteria relies on the recognition of specific forms of peptidoglycan.
引用
收藏
页码:478 / 484
页数:7
相关论文
共 43 条
[1]   Toll-like receptors: critical proteins linking innate and acquired immunity [J].
Akira, S ;
Takeda, K ;
Kaisho, T .
NATURE IMMUNOLOGY, 2001, 2 (08) :675-680
[2]   ACTIVATION OF PRO-PHENOLOXIDASE BY BACTERIAL-CELL WALLS OR BETA-1,3-GLUCANS IN PLASMA OF THE SILKWORM, BOMBYX-MORI [J].
ASHIDA, M ;
ISHIZAKI, Y ;
IWAHANA, H .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1983, 113 (02) :562-568
[3]  
Aussel L, 2000, RAPID COMMUN MASS SP, V14, P595, DOI 10.1002/(SICI)1097-0231(20000415)14:7<595::AID-RCM919>3.0.CO
[4]  
2-4
[5]   Sequential activation of signaling pathways during innate immune responses in Drosophila [J].
Boutros, M ;
Agaisse, H ;
Perrimon, N .
DEVELOPMENTAL CELL, 2002, 3 (05) :711-722
[6]   Requirement for a peptidoglycan recognition protein (PGRP) in relish activation and antibacterial immune responses in Drosophila [J].
Choe, KM ;
Werner, T ;
Stöven, S ;
Hultmark, D ;
Anderson, KV .
SCIENCE, 2002, 296 (5566) :359-362
[7]   One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products [J].
Datsenko, KA ;
Wanner, BL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (12) :6640-6645
[8]   The Toll and Imd pathways are the major regulators of the immune response in Drosophila [J].
De Gregorio, E ;
Spellman, PT ;
Tzou, P ;
Rubin, GM ;
Lemaitre, B .
EMBO JOURNAL, 2002, 21 (11) :2568-2579
[9]   Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection [J].
Girardin, SE ;
Boneca, IG ;
Viala, J ;
Chamaillard, M ;
Labigne, A ;
Thomas, G ;
Philpott, DJ ;
Sansonetti, PJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (11) :8869-8872
[10]  
GLAUNER B, 1988, J BIOL CHEM, V263, P10088