Sequence conservation within the major capsid protein of human papillomavirus (HPV) type 18 and formation of HPV-18 virus-like particles in Saccharomyces cerevisiae

被引:41
作者
Hofmann, KJ
Neeper, MP
Markus, HZ
Brown, DR
Muller, M
Jansen, KU
机构
[1] MERCK SHARP & DOHME LTD,DEPT VIRUS & CELL BIOL,W POINT,PA 19486
[2] INDIANA UNIV,SCH MED,DIV INFECT DIS,INDIANAPOLIS,IN 46202
[3] RICHARD L ROUDEBUSH VET ADM MED CTR,INDIANAPOLIS,IN 46202
[4] LOYOLA UNIV,MED CTR,MAYWOOD,IL 60153
关键词
D O I
10.1099/0022-1317-77-3-465
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The major capsid protein L1 of human papillomaviruses (HPVs) has been identified as a promising candidate antigen for a prophylactic HPV vaccine. Since amino acid sequence heterogeneity has been demonstrated for the L1 genes within individual HPV types, nucleotide sequences for L1 were determined from six HPV-18 clinical isolates and the cervical carcinoma cell line SW756 and compared to the published HPV-18 prototype sequence. The sequences were almost identical between the clinical isolates and SW756 but differed markedly from the published prototype sequence. Resequencing the prototype HPV-18 revealed that these differences were due to sequencing artifacts of the prototype HPV-18 sequence archived in GenBank. Thus, the HPV-18 L1 genes seem to display a very high level of sequence conservation. The HPV-18 L1 gene derived from SW756 was expressed in Saccharomyces cerevisiae and self-assembly of the L1 protein into viruslike particles was demonstrated.
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收藏
页码:465 / 468
页数:4
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