Could light meal jeopardize laboratory coagulation tests?

被引:22
作者
Lima-Oliveira, Gabriel [1 ,2 ,3 ,4 ]
Salvagno, Gian Luca [1 ]
Lippi, Giuseppe [5 ]
Danese, Elisa [1 ]
Gelati, Matteo [1 ]
Montagnana, Martina [1 ]
Picheth, Geraldo [2 ]
Guidi, Gian Cesare [1 ,2 ]
机构
[1] Univ Verona, Dept Life & Reprod Sci, Lab Clin Biochem, I-37100 Verona, Italy
[2] Univ Fed Parana, Dept Clin Anal, Postgrad Program Pharmaceut Sci, BR-80060000 Curitiba, Parana, Brazil
[3] Minist Educ Brazil, CAPES Fdn, Brasilia, DF, Brazil
[4] MERCOSUL Sect Comm Clin Anal & Vitro Diag CSM 20, Rio De Janeiro, Brazil
[5] Acad Hosp Parma, Lab Clin Chem & Hematol, Parma, Italy
关键词
diagnostic errors; fasting; postprandial period; reference values; reproducibility of results; PREANALYTICAL VARIABILITY; BIOLOGICAL VARIATION; VACUUM TUBES; COLLECTION; RECOMMENDATIONS; BIOCHEMISTRY; TOURNIQUET; QUALITY; STASIS; ORDER;
D O I
10.11613/BM.2014.036
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Presently the necessity of fasting time for coagulation tests is not standardized. Our hypothesis is that this can harm patient safety. This study is aimed at evaluating whether a light meal (i.e. breakfast) can jeopardize laboratory coagulation tests. Materials and methods: A blood sample was firstly collected from 17 fasting volunteers (12 h). Immediately after blood collection, the volunteers consumed a light meal. Then samples were collected at 1,2 and 4 h after the meal. Coagulation tests included: activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (Fbg), antithrombin III (AT), protein C (PC) and protein S (PS). Differences between samples were assessed by Wilcoxon ranked-pairs test. The level of statistical significance was set at P < 0.05. Mean % differences were determined and differences between and baseline and 1,2 and 4h samples were compared with reference change value (RCV). Results: A significantly higher % activity of AT was observed at 1 h and 4 h after meal vs. baseline specimen [113 (104-117) and 111 (107-120) vs. 109 (102-118), respectively; P = 0.029 and P = 0.016]. APTT at 2 h was found significantly lower than baseline samples [32.0 (29.9-34.8) vs. 34.1 (32.2-35.2), respectively; P = 0.041]. The results of both Fbg and PS tests were not influenced by a light meal. Furthermore, no coagulation tests had significant variation after comparison with RCV. Conclusion: A light meal does not influence the laboratory coagulation tests we assessed, but we suggest that the laboratory quality managers standardize the fasting time for all blood tests at 12 hours, to completely metabolize the lipids intake.
引用
收藏
页码:343 / 349
页数:7
相关论文
共 30 条
[1]  
Clinical and Laboratory Standards Institute, 2010, H18A4 CLSI
[2]  
Clinical and Laboratory Standards Institute, 2007, H3A6 CLSI
[3]   The cost of poor blood specimen quality and errors in preanalytical processes [J].
Green, Sol F. .
CLINICAL BIOCHEMISTRY, 2013, 46 (13-14) :1175-1179
[4]  
Guder WG., 2009, DIAGNOSTIC SAMPLES P
[5]   Elimination of the venous stasis error for routine coagulation testing by transillumination [J].
Lima-Oliveira, G. ;
Salvagno, G. L. ;
Lippi, G. ;
Montagnana, M. ;
Scartezini, M. ;
Picheth, G. ;
Guidi, G. C. .
CLINICA CHIMICA ACTA, 2011, 412 (15-16) :1482-1484
[6]  
Lima-Oliveira G, 2014, J J MED BIOCH
[7]   Processing of Diagnostic Blood Specimens: Is It Really Necessary to Mix Primary Blood Tubes after Collection with Evacuated Tube System? [J].
Lima-Oliveira, Gabriel ;
Lippi, Giuseppe ;
Salvagno, Gian Luca ;
Brocco, Giorgio ;
Gaino, Stefania ;
Dima, Francesco ;
Volaski, Waldemar ;
Rego, Fabiane Gomes ;
Picheth, Geraldo ;
Guidi, Gian Cesare .
BIOPRESERVATION AND BIOBANKING, 2014, 12 (01) :53-59
[8]  
Lima-Oliveira G, 2013, BIOCHEM MEDICA, V23, P308
[9]   Incorrect order of draw could be mitigate the patient safety: a phlebotomy management case report [J].
Lima-Oliveira, Gabriel ;
Lippi, Giuseppe ;
Salvagno, Gian Luca ;
Montagnana, Martina ;
Picheth, Geraldo ;
Guidi, Gian Cesare .
BIOCHEMIA MEDICA, 2013, 23 (02) :218-223
[10]   Sodium citrate vacuum tubes validation: preventing preanalytical variability in routine coagulation testing [J].
Lima-Oliveira, Gabriel ;
Lippi, Giuseppe ;
Salvagno, Gian Luca ;
Montagnana, Martina ;
Picheth, Geraldo ;
Guidi, Gian Cesare .
BLOOD COAGULATION & FIBRINOLYSIS, 2013, 24 (03) :252-255