Indium-labeled white blood cells apheresed from donors receiving G-CSF localize to sites of inflammation when infused into allogeneic bone marrow transplant recipients

被引:42
作者
Adkins, D
Goodgold, H
Hendershott, L
Johnston, M
Cravens, D
Spitzer, G
机构
[1] ST LOUIS UNIV,HLTH SCI CTR,DEPT INTERNAL MED,ST LOUIS,MO 63103
[2] LDS HOSP,CLIN TRIALS OFF,INTERMT HEALTHCARE INC,SALT LAKE CITY,UT
关键词
granulocyte transfusions; G-CSF; indium labeled;
D O I
10.1038/sj.bmt.1700749
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
G-CSF administration to normal donors results in granulocyte apheresis yields generally greater than those observed with other neutrophil mobilizing agents, In vitro, neutrophils cultured with G-CSF exhibit prolonged survival; however, the random migration of neutrophils exposed to this agent is inhibited, Although transfused neutrophils mobilized with agents other than G-CSF migrate to sites of inflammation or infection in vivo, this has yet to be demonstrated with infusion of G-CSF-mobilized neutrophils into neutropenic human subjects, Five neutropenic allogeneic bone marrow transplant (BMT) patients each received a fresh infusion of G-CSF-mobilized indium-labeled irradiated white blood cells (WBC) apheresed from HLA-matched normal donors on day +5 post-transplant. Localization of activity on delayed scintigraphic images of indium-labeled WBC scans to sites of tissue damage (oral/nasopharynx in two patients with mucositis and terminal ileum/cecum in one with diarrhea) occurred, and supports the hypothesis that G-CSF-mobilized HLA-matched donor neutrophils which have been irradiated are functional after infusion into neutropenic recipients.
引用
收藏
页码:809 / 812
页数:4
相关论文
共 23 条
[1]  
Adkins D, 1995, BLOOD, V86, P2317
[2]  
APPELBAUM FR, 1977, BLOOD, V49, P483
[3]   CONSEQUENCES OF PRIOR ALLOIMMUNIZATION DURING GRANULOCYTE TRANSFUSION [J].
APPELBAUM, FR ;
TRAPANI, RJ ;
GRAW, RG .
TRANSFUSION, 1977, 17 (05) :460-464
[4]  
BEGLEY CG, 1986, BLOOD, V68, P162
[5]   THE EFFECTS OF DAILY RECOMBINANT HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR ADMINISTRATION ON NORMAL GRANULOCYTE DONORS UNDERGOING LEUKAPHERESIS [J].
BENSINGER, WI ;
PRICE, TH ;
DALE, DC ;
APPELBAUM, FR ;
CLIFT, R ;
LILLEBY, K ;
WILLIAMS, B ;
STORB, R ;
THOMAS, ED ;
BUCKNER, CD .
BLOOD, 1993, 81 (07) :1883-1888
[6]   EFFECTIVE STIMULATION OF DONORS FOR GRANULOCYTE TRANSFUSIONS WITH RECOMBINANT METHIONYL GRANULOCYTE-COLONY-STIMULATING FACTOR [J].
CASPAR, CB ;
SEGER, RA ;
BURGER, J ;
GMUR, J .
BLOOD, 1993, 81 (11) :2866-2871
[7]  
CHOW HS, 1980, BLOOD, V55, P546
[8]  
COLOTTA F, 1992, BLOOD, V80, P2012
[9]  
COLOTTA F, 1993, BLOOD, V81, P2258
[10]  
DUTCHER JP, 1983, BLOOD, V62, P354