Divergent effects of estrogen and nicotine on Rho-kinase expression in human coronary vascular smooth muscle cells

被引:57
作者
Hiroki, J [1 ]
Shimokawa, H [1 ]
Mukai, Y [1 ]
Ichiki, T [1 ]
Takeshita, A [1 ]
机构
[1] Kyushu Univ, Grad Sch Med Sci, Dept Cardiovasc Med, 21st Century COE Program Lifestyle Related Dis, Fukuoka, Japan
关键词
Rho-kinase; nicotine; estrogen; estrogen receptor; vascular smooth muscle; coronary arteriosclerosis;
D O I
10.1016/j.bbrc.2004.11.011
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent studies have demonstrated that up-regulated Rho-kinase plays an important role in the pathogenesis of coronary arteriosclerosis and vasospasm. We have shown that inflammatory stimuli, such as angiotensin II and interleukin-1beta, up-regulate Rho-kinase expression and activity in human coronary vascular smooth muscle cells, for which intracellular signal transduction mediated by protein kinase C and NF-kappaB is involved. Here, we show that estrogen down-regulates while nicotine up-regulates Rho-kinase and that nicotine counteracts the inhibitory effect of estrogen on angiotensin II-induced Rho-kinase expression. Furthermore, we demonstrated that the intracellular signal transduction of the inhibitory effect of estrogen is mediated by an estrogen receptor. These results demonstrate that inflammatory stimuli up-regulate Rho-kinase, for which estrogen (mediated by an estrogen receptor) and nicotine exert divergent inhibitory and stimulatory effects on the Rho-kinase expression, respectively, and may explain in part why the incidence of arteriosclerotic and vasospastic disorders is increased in postmenopausal women and smokers. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:154 / 159
页数:6
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