Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol

被引：287

作者：

Li, Yantao ^{[1
]}

Han, Danxiang ^{[1
]}

Hu, Guongrong ^{[1
]}

Dauvillee, David ^{[2
]}

Sommerfeld, Milton ^{[1
]}

Ball, Steven ^{[2
]}

Hua, Qiang ^{[1
]}

机构：

[1] Arizona State Univ, LARB, Mesa, AZ 85212 USA

[2] Univ Lille 1, CNRS, UMR 8576, UGSF, F-59655 Villeneuve Dascq, France

来源：

METABOLIC ENGINEERING | 2010年 / 12卷 / 04期

关键词：

Biofuel; High light; Nitrogen starvation; Oleaginous microalgae; Starch; Triacylglycerol; ALPHA-1,4 GLUCANOTRANSFERASES; REINHARDTII; FEEDSTOCKS; BIOFUELS; ALGAE;

D O I：

10.1016/j.ymben.2010.02.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Many microalgae and plants have the ability to synthesize large amounts of triacylglycerol (TAG) that can be used to produce biofuels. Presently, TAG-based biofuel production is limited by the feedstock supply. Metabolic engineering of lipid synthesis pathways to overproduce TAGs in oleaginous microalgae and oil crop plants has achieved only modest success. We demonstrate that inactivation of ADP-glucose pyrophosphorylase in a Chlamydomonas strachless mutant led to a 10-fold increase in TAG, suggesting that shunting of photosynthetic carbon partitioning from starch to TAG synthesis may represent a more effective strategy than direct manipulation of the lipid synthesis pathway to overproduce TAG. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：387 / 391

页数：5

共 24 条

[1]

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