High-resolution mapping adjacent to the Pc71 crown-rust resistance locus in hexaploid oat

被引:32
作者
Bush, AL
Wise, RP [1 ]
机构
[1] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA
[2] Iowa State Univ, Dept Plant Pathol, Ames, IA 50011 USA
[3] Iowa State Univ, Interdept Genet Program, Ames, IA 50011 USA
关键词
bacterial artificial chromosome (BAC); Gramineae; oat crown rust; Puccinia coronata; RFLP mapping;
D O I
10.1023/A:1009652222382
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The D526-derived BC1F2 population of hexaploid oat segregates for resistance to crown rust isolate 345. A mapping population consisting of 440 F2 individuals was used to develop a high-resolution RFLP map of the area of the genome where Pc71, the locus conferring this resistance, is located. Twelve RFLP markers have been identified within ca. 6 cM of Pc71, with cosegregating loci Xcdo1502 and Xcdo783 positioned 0.2 cM from the locus. All of the RFLP markets map to the same side of the locus, suggesting either that the Pc71 resistance locus resides at the end of a linkage group, or that there is no detectable heterozygosity on the opposite side. Some degree of microcollinearity between lice and oat is present in this area, as the two markers, Xcdo783 and Xrz69, linked to Pc71 are linked also in rice; however the genetic distance in oat is much less than the genetic distance in rice (6.2 cM and 20 cM, respectively).
引用
收藏
页码:13 / 21
页数:9
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