A conserved IL-2 responsive enhancer in the IL-2Rα gene

被引：7

作者：

Bucher, P

Corthésy, P

Imbert, J

Nabholz, M

机构：

[1] Swiss Inst Expt Canc Res, CH-1066 Epalinges, Switzerland

[2] Inst J Paoli I Calmettes, INSERM U119, F-13009 Marseille, France

来源：

IMMUNOBIOLOGY | 1997年 / 198卷 / 1-3期

关键词：

D O I：

10.1016/S0171-2985(97)80034-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IL-2 stimulates expression of the alpha subunit of the high affinity IL-2 receptor (IL-2R alpha) in antigen-activated T lymphocytes, by increasing IL-2R alpha gene transcription. This response is mediated by a 52 nt IL-2 responsive enhancer (IL-2rE) that is conserved between mouse and man. The mouse enhancer is 1.3 kb upstream of the transcription start site and co-localizes with an inducible DNasel hypersensitive site, whereas the human homologue maps to -4 kb. The human IL-2rE is functional in rodent cells. Both enhancers contain two potential STAT binding sites and an Ets consensus motif, One of the STAT motifs overlaps with a binding site for GATA factors. Functional analysis of the mouse and human enhancers indicates that IL-2-activated STAT5 and the constitutively active Ets protein Elf-1 play a predominant role in controlling IL-2rE activity.

引用

页码：136 / 143

页数：8

共 20 条

[1] RECONSTITUTION OF FUNCTIONAL INTERLEUKIN-2 RECEPTOR COMPLEXES ON FIBROBLASTOID CELLS - INVOLVEMENT OF THE CYTOPLASMIC DOMAIN OF THE GAMMA-CHAIN IN 2 DISTINCT SIGNALING PATHWAYS [J].