Isolation and screening for alkaline thermostable xylanases

被引：19

作者：

Subramaniyan, S ^{[1
]}

Prema, P ^{[1
]}

Ramakrishna, SV ^{[1
]}

机构：

[1] CSIR, Reg Res Lab, Biochem Proc & Wastewater Technol, Trivandrum 695019, Kerala, India

来源：

JOURNAL OF BASIC MICROBIOLOGY | 1997年 / 37卷 / 06期

关键词：

THERMOPHILIC BACILLUS SP; STRAIN; PURIFICATION;

D O I：

10.1002/jobm.3620370610

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Screening of xylanase producing microorganisms from soil samples and hemicellulosic materials were carried out in three stages using wheat bran extract agar medium, xylan agar medium and liquid medium with xylan. One of the isolates, identified as Bacillus SSP-34 produced 100 times more xylanase activity than the other isolates. Maximum enzyme production by this isolate was observed after 102 hours of growth. The optimum pH for the crude xylanase preparation range from 6-8 and optimum temperature was at 50 degrees C. The enzyme was stable up to 20 minutes at 50 degrees C and at pH range 4.5-9.0 for more than 2 hours.

引用

页码：431 / 437

页数：7

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