Improving draft assemblies by iterative mapping and assembly of short reads to eliminate gaps

被引：225

作者：

Tsai, Isheng J. ^{[1
]}

Otto, Thomas D. ^{[1
]}

Berriman, Matthew ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

来源：

GENOME BIOLOGY | 2010年 / 11卷 / 04期

关键词：

SEQUENCE DATA; PROGRAM;

D O I：

10.1186/gb-2010-11-4-r41

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Advances in sequencing technology allow genomes to be sequenced at vastly decreased costs. However, the assembled data frequently are highly fragmented with many gaps. We present a practical approach that uses Illumina sequences to improve draft genome assemblies by aligning sequences against contig ends and performing local assemblies to produce gap-spanning contigs. The continuity of a draft genome can thus be substantially improved, often without the need to generate new data.

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页数：9

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