Shotgun cloning and characterization of the thymidylate synthase-encoding gene from Mycobacterium bovis BCG

被引：1

作者：

Matsumoto, S

Yukitake, H

Ohara, N

Dairi, T

Kanbara, H

Yamada, T

机构：

[1] Nagasaki Univ, Sch Dent, Nagasaki 852, Japan

[2] Toyama Prefectural Univ, Biotechnol Res Ctr, Toyama 93903, Japan

[3] Inst Trop Med, Dept Protozool, Nagasaki 852, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1998年 / 42卷 / 01期

关键词：

mycobacterial enzyme; thyA; gene expression;

D O I：

10.1111/j.1348-0421.1998.tb01964.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The shotgun cloning of a Mycobacterium bovis BCG (BCG) genome into pBluescript SK (+) successfully yielded a 0.9 kbp fragment, confirming the ability of Escherichia coli thyA mutant MH2702 to grow in a thymine-depleted medium. This DNA fragment contained a gene homologous to the thymidylate synthase (TS)-encoding genes (thyA) of other organisms. An inverted repeat sequence and open reading frame (ORF) were observed at the upstream region of the thyA. A computer analysis revealed that the protein encoded by this ORF possessed a structure unique for a DNA binding protein.

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页码：15 / 21

页数：7

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