Comparison between Haemophilus parasuis infection in colostrums-deprived and sow-reared piglets

被引:50
作者
Blanco, I
Galina-Pantoja, L
Oliveira, S
Pijoan, C
Sánchez, C
Canals, A
机构
[1] Ctr Invest Sanidad Anim, Madrid 28130, Spain
[2] Sygen Int, PIC, Franklin, KY 42135 USA
[3] Univ Minnesota, Dept Clin & Populat Sci, St Paul, MN 55108 USA
[4] Agencia Espanola Medicamento, Madrid 28071, Spain
关键词
pig-bacteria; Glasser's disease; colostrum-deprived; infection models; Haemophilus parasuis;
D O I
10.1016/j.vetmic.2004.06.011
中图分类号
Q93 [微生物学];
学科分类号
071005 [微生物学]; 100705 [微生物与生化药学];
摘要
The aim of this study was to compare the development of Glasser's disease in sow-reared and colostrum-deprived piglets. Ninety piglets from a commercial pig farm in Spain were used. The farm was positive for Haemophilus parasuis. Fifty-two pigs were sow-reared (SR) and 38 were colostrum-deprived (CD) piglets. The animals were intratracheally inoculated with H. parasuis serovar 5 and sacrificed at 1, 2 and 3 days post-infection. To assess the development of disease, antibody titers, clinical signs, pathological lesions, microbiological isolation and PCR amplification were compared between the groups. Inoculation of SR pigs did not cause clinical signs or lesions of Glasser's disease. In SR pigs, H. parasuis isolation and specific PCR amplification from tissues showed a very low number of positive samples. In contrast, in CD pigs, inoculation resulted in the typical signs and lesions of Glasser's disease. Positive microbiological isolation and specific PCR products were obtained from the majority of the tissues tested, and no antibodies against H. parasuis were detected. The experimental infection using CD pigs describes a successful method to study this microorganism and confirms the important role that maternal antibodies play in protection against clinical signs and disease. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:21 / 27
页数:7
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