Characterization of a culture method to recover Helicobacter pylori from the feces of infected patients

被引:33
作者
Dore, MP
Osato, MS
Malaty, HM
Graham, DY
机构
[1] Vet Affairs Med Ctr, Houston, TX 77030 USA
[2] Baylor Coll Med, Houston, TX 77030 USA
关键词
D O I
10.1046/j.1523-5378.2000.00026.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background. Helicobacter pylori is difficult to culture from stool. Multiple efforts from multiple laboratories have been unsuccessful, and the optimal conditions to recover H. pylori from stool are still not known. Recovery of H. pylori from feces of infected individuals is important for the performance of molecular epidemiological investigations, especially in children, where their symptoms do not warrant endoscopy to recover the organism. Methods. Fresh fecal specimens (noncathartic) were obtained from 19 known H. pylori-infected patients and were processed to recover the organism. Fresh fecal specimens (noncathartic) were also obtained from three known H. pylori-negative individuals (controls) to determine whether H. pylori could be isolated from stools seeded with known concentrations of the organism. Treatment of the fecal suspensions with cholestyramine, a basic anion exchange resin that binds bile acids, was used in an attempt to enhance recovery of H. pylori by sequestering bile acids that are inhibitory to H. pylori growth. H. pylori was identified based on colony morphology, cell morphology, Gram's stain, biochemical reactions, and polymerase chain reaction for two H. pylori genes. Results. Among 19 patients, H. pylori was cultured at least once from 3 and three times from 2 (5 of 19). Feces that were seeded with H. pylori and obtained from three H. pylori-negative volunteer controls yielded positive recovery in all instances. Conclusion. We have confirmed that it is possible to culture H. pylori from human stool, but the procedure for optimal recovery has still not been defined.
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页码:165 / 168
页数:4
相关论文
共 18 条
[1]  
Chang MC, 1999, HEPATO-GASTROENTEROL, V46, P299
[2]   Helicobacter pylori in sheep milk [J].
Dore, MP ;
Sepulveda, AR ;
Osato, MS ;
Realdi, G ;
Graham, DY .
LANCET, 1999, 354 (9173) :132-132
[3]   CAMPYLOBACTER-PYLORI COLONIZING HETEROTOPIC GASTRIC TISSUE IN THE RECTUM [J].
DYE, KR ;
MARSHALL, BJ ;
FRIERSON, HF ;
PAMBIANCO, DJ ;
MCCALLUM, RW .
AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1990, 93 (01) :144-147
[4]   REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION AMPLIFICATION OF RIBOSOMAL-RNA FOR DETECTION OF HELICOBACTER SPECIES [J].
ENGSTRAND, L ;
NGUYEN, AMH ;
GRAHAM, DY ;
ELZAATARI, FAK .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (09) :2295-2301
[5]   IMMUNOMAGNETIC SEPARATION AND PCR FOR DETECTION OF HELICOBACTER-PYLORI IN WATER AND STOOL SPECIMENS [J].
ENROTH, H ;
ENGSTRAND, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (08) :2162-2165
[6]  
Graham DY, 2000, AM J GASTROENTEROL, V95, P87, DOI 10.1111/j.1572-0241.2000.01704.x
[7]   Antimicrobial susceptibility testing of Helicobacter pylori - Comparison of E-test, broth microdilution, and disk diffusion for ampicillin, clarithromycin, and metronidazole [J].
Hachem, CY ;
Clarridge, JE ;
Reddy, R ;
Flamm, R ;
Evans, DG ;
Tanaka, K ;
Graham, DY .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1996, 24 (01) :37-41
[8]   SEROPREVALENCE OF HELICOBACTER-PYLORI IN CHILE - VEGETABLES MAY SERVE AS ONE ROUTE OF TRANSMISSION [J].
HOPKINS, RJ ;
VIAL, PA ;
FERRECCIO, C ;
OVALLE, J ;
PRADO, P ;
SOTOMAYOR, V ;
RUSSELL, RG ;
WASSERMAN, SS ;
MORRIS, JG .
JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (01) :222-226
[9]   ISOLATION OF HELICOBACTER-PYLORI FROM FECES OF PATIENTS WITH DYSPEPSIA IN THE UNITED-KINGDOM [J].
KELLY, SM ;
PITCHER, MCL ;
FARMERY, SM ;
GIBSON, GR .
GASTROENTEROLOGY, 1994, 107 (06) :1671-1674
[10]   WATER SOURCE AS RISK FACTOR FOR HELICOBACTER-PYLORI INFECTION IN PERUVIAN CHILDREN [J].
KLEIN, PD ;
GRAHAM, DY ;
GAILLOUR, A ;
OPEKUN, AR ;
SMITH, EO .
LANCET, 1991, 337 (8756) :1503-1506