Intercalation, delivery, and expression of the gene encoding green fluorescence protein utilizing nanobiohybrids

被引：77

作者：

Tyner, KM

Roberson, MS

Berghorn, KA

Li, L

Gilmour, RF

Batt, CA

Giannelis, EP ^{[1
]}

机构：

[1] Cornell Univ, Dept Mat Sci & Engn, Ithaca, NY 14853 USA

[2] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

[3] Cornell Univ, Dept Biomed Sci, Ithaca, NY 14853 USA

来源：

JOURNAL OF CONTROLLED RELEASE | 2004年 / 100卷 / 03期

关键词：

layered double hydroxide; nanobiohybrid; non-viral vector;

D O I：

10.1016/j.jconrel.2004.07.035

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Non-viral gene vectors have attracted much attention recently due to setbacks with viral delivery systems. Here a new non-viral delivery system based on nanobiohybrids synthesized by the intercalation of a full gene and promoter encoding Green Fluorescent Protein (GFP) between the layers of an inorganic host is reported. The nanobiohybrids were delivered to 9L glioma cells, JEG3 choriocarcinoma placental cells, and cardiac myocytes. All cells were able to internalize and tolerate the nanobiohybrids. In addition, all cells expressed the gene with some cell lines having up to 90% transfection efficiency. This new, bio-mimetic delivery system shows promise for use in non-viral gene therapy. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：399 / 409

页数：11

共 26 条

[1] Effect of hydrotalcite-like compounds on the aqueous solubility of some poorly water-soluble drugs [J].