A novel multifunctional triterpene synthase from Arabidopsis thaliana

被引:68
作者
Kushiro, T
Shibuya, M
Masuda, K
Ebizuka, Y
机构
[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Showa Pharmaceut Univ, Machida, Tokyo 1948543, Japan
基金
日本学术振兴会;
关键词
Arabidopsis thaliana; YUP8H12R.43; oxidosqualene cyclase; triterpene synthase;
D O I
10.1016/S0040-4039(00)01347-2
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The Arabiciopsis thaliana genome sequencing project has identified several triterpene synthase homologues. One cDNA of these clones, YUP8H12R.43, was obtained and functionally expressed in yeast. At least nine triterpenes have been identified as its products based on NMR and LCMS analysis. The products include extensively migrated triterpenes, multiflorenol and bauerenol. Several chimeric clones were constructed between YUP8H12R.43 and A. thaliana lupeol synthase LUP1, to reveal that a C-terminal half and a part of N-terminus is important for such product multiplicity. The presence of such multifunctional triterpene synthase in plants is noteworthy from both a mechanistic and a physiological point of view. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:7705 / 7710
页数:6
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