Role of protein kinase C in the release of [3H]acetylcholine from myenteric plexus treated with vesamicol

被引：8

作者：

Clarizia, AD ^{[1
]}

Romano-Silva, MA ^{[1
]}

Prado, VF ^{[1
]}

Gomez, MV ^{[1
]}

Prado, MAM ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Farmacol Bioquim Imunol, Lab Neurofarmacol, BR-30161 Belo Horizonte, MG, Brazil

来源：

NEUROSCIENCE LETTERS | 1998年 / 244卷 / 02期

关键词：

acetylcholine release; vesamicol; vesicular acetylcholine transporter; synaptic vesicles; protein kinase C; ouabain;

D O I：

10.1016/S0304-3940(98)00143-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

The present experiments investigated the release of [3H]acetylcholine ([H-3]ACh) from the guinea pig myenteric plexus treated with 2-(4-phenylpiperidino)cyclohexanol (vesamicol), a drug that impairs ACh accumulation by synaptic vesicles. Ouabain, an Na+-K+ ATPase inhibitor, released [3H]ACh synthesised in the presence of (-)-vesamicol, while electrical field stimulation or KCl depolarisation were not effective to release the transmitter in this condition. The effect of ouabain was Ca2+-dependent and in the presence of (-)-vesamicol it was blocked by calphostin C, an inhibitor of protein kinase C (PKC). In addition, stimulation of kinase C activity by a phorbol ester, but not by its inactive isomer, prevented (-)-vesamicol from interfering with the release of [H-3]ACh in electrically-stimulated myenteric plexus, similar to the effect of ouabain. We conclude that release of [H-3]ACh induced by ouabain in the presence of (-)-vesamicol depends on PKC activation. (C) 1998 Published by Elsevier Science Ireland Ltd.

引用

页码：115 / 117

页数：3

共 11 条

[1]

EXTERNAL CA2+-INDEPENDENT RELEASE OF NEUROTRANSMITTERS [J].