Combinatorial modification of multiple lignin traits in trees through multigene cotransformation

被引:302
作者
Li, L
Zhou, YH
Cheng, XF
Sun, JY
Marita, JM
Ralph, J
Chiang, VL [1 ]
机构
[1] Michigan Technol Univ, Sch Forestry & Wood Prod, Houghton, MI 49931 USA
[2] Univ Wisconsin, USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA
[3] Univ Wisconsin, Agr Res Serv, Madison, WI 53706 USA
[4] Univ Wisconsin, Dept Forestry, Madison, WI 53706 USA
关键词
D O I
10.1073/pnas.0831166100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Lignin quantity and reactivity [which is associated with its syringyl/guaiacyl (S/G) constituent ratio] are two major barriers to wood-pulp production. To verify our contention that these traits are regulated by distinct monolignol biosynthesis genes, encoding 4-coumarate-CoA ligase (4CL) and coniferaldehyde 5-hydroxylase (CAld5H), we used Agrobacterium to cotransfer antisense 4CL and sense CAId5H genes into aspen (Populus tremuloides). Trees expressing each one and both of the transgenes were produced with high efficiency. Lignin reduction by as much as 40% with 14% cellulose augmentation was achieved in antisense 4CL plants; S/G-ratio increases as much as 3-fold were observed without lignin quantity change in sense CAId5H plants. Consistent with our contention, these effects were independent but additive, with plants expressing both transgenes having up to 52% less lignin, a 64% higher S/G ratio, and 30% more cellulose. An S/G-ratio increase also accelerated cell maturation in stem secondary xylem, pointing to a role for syringyl lignin moieties in coordinating xylem secondary wall biosynthesis. The results suggest that this multigene cotransfer system should be broadly useful for plant genetic engineering and functional genomics.
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页码:4939 / 4944
页数:6
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