Development of Agrobacterium tumefaciens C58-induced plant tumors and impact on host shoots are controlled by a cascade of jasmonic acid, auxin, cytokinin, ethylene and abscisic acid

被引:62
作者
Veselov, D
Langhans, M
Hartung, W
Aloni, R
Feussner, I
Götz, C
Veselova, S
Schlomski, S
Dickler, C
Bächmann, K
Ullrich, CI
机构
[1] Tech Univ Darmstadt, Inst Bot, D-64287 Darmstadt, Germany
[2] Inst Biol, Ufa 450054, Russia
[3] Univ Wurzburg, J V Sachs Inst Biowissenschaften, D-97082 Wurzburg, Germany
[4] Tel Aviv Univ, Dept Plant Sci, IL-69978 Tel Aviv, Israel
[5] IPK, D-06466 Gatersleben, Germany
[6] Inst Pflanzenbiochem, D-06120 Halle An Der Saale, Germany
[7] Tech Univ Darmstadt, D-64287 Darmstadt, Germany
关键词
abscisic acid; Agrobacterium tumefaciens-induced tumor; auxin and cytokinin immunolocalization; ethylene; jasmonic acid; Ricinus;
D O I
10.1007/s00425-002-0883-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The development of Agrobacterium tumefaciens-induced plant tumors primarily depends on the excessive production of auxin and cytokinin by enzymes encoded on T-DNA genes integrated into the plant genome. The aim of the present study was to investigate the involvement of additional phytohormone signals in the vascularization required for rapid tumor proliferation. In stem tumors of Ricinus communis L., free auxin and zeatin riboside concentrations increased within 2 weeks to 15-fold the concentrations in control stem tissue. Auxin and cytokinin immunolocalization revealed the highest concentrations within and around tumor vascular bundles with concentration gradients. The time-course of changes in free auxin concentration in roots was inversely correlated with that in the tumors. The high ethylene emission induced by increased auxin- and cytokinin correlated with a 36-fold accumulation of abscisic acid in tumors. Ethylene emitted from tumors and exogenously applied ethylene caused an increase in abscisic acid concentrations also in the host leaves, with a diminution in leaf water vapor conductance. Jasmonic acid concentration reached a maximum already within the first week of bacterial infection. A wound effect could be excluded. The results demonstrate the concerted interaction of a cascade of transiently induced, non-TDNA-encoded phytohormones jasmonic acid, ethylene and abscisic acid with T-DNA-encoded auxin and zeatin riboside plus trans-zeatin, all of which are required for successful plant tumor vascularization and development together with inhibition of host plant growth.
引用
收藏
页码:512 / 522
页数:11
相关论文
共 43 条
[1]   The Never ripe mutant provides evidence that tumor-induced ethylene controls the morphogenesis of Agrobacterium tumefaciens-induced crown galls on tomato stems [J].
Aloni, R ;
Wolf, A ;
Feigenbaum, P ;
Avni, A ;
Klee, HJ .
PLANT PHYSIOLOGY, 1998, 117 (03) :841-849
[2]  
ALONI R, 1995, PLANTA, V196, P597, DOI 10.1007/BF00203661
[3]   Foliar and axial aspects of vascular differentiation: Hypotheses and evidence [J].
Aloni, R .
JOURNAL OF PLANT GROWTH REGULATION, 2001, 20 (01) :22-34
[4]  
Aloni Roni, 1995, P531
[5]  
[Anonymous], 1991, ABSCISIC ACID PHYSL
[6]   In situ localization of endogenous cytokinins during shooty tumor development on Eucalyptus globulus Labill. [J].
Azmi, A ;
Dewitte, W ;
Van Onckelen, H ;
Chriqui, D .
PLANTA, 2001, 213 (01) :29-36
[7]   Inputs to the active indole-3-acetic acid pool:: De novo synthesis, conjugate hydrolysis, and indole-3-butyric acid β-oxidation [J].
Bartel, B ;
LeClere, S ;
Magidin, M ;
Zolman, BK .
JOURNAL OF PLANT GROWTH REGULATION, 2001, 20 (03) :198-216
[8]   Interactions between abscisic acid and ethylene signaling cascades [J].
Beaudoin, N ;
Serizet, C ;
Gosti, F ;
Giraudat, J .
PLANT CELL, 2000, 12 (07) :1103-1115
[9]   Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer:: role of phytohormones [J].
Chateau, S ;
Sangwan, RS ;
Sangwan-Norreel, BS .
JOURNAL OF EXPERIMENTAL BOTANY, 2000, 51 (353) :1961-1968
[10]  
CLELAND RE, 1999, BIOCH MOL BIOL PLANT, P10