The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

被引：342

作者：

Fritz-Laylin, Lillian K. ^{[1
]}

Prochnik, Simon E. ^{[3
]}

Ginger, Michael L. ^{[4
]}

Dacks, Joel B. ^{[5
,6
]}

Carpenter, Meredith L. ^{[1
]}

Field, Mark C. ^{[6
]}

Kuo, Alan ^{[3
]}

Paredez, Alex ^{[1
]}

Chapman, Jarrod ^{[3
]}

Pham, Jonathan ^{[7
]}

Shu, Shengqiang ^{[3
]}

Neupane, Rochak ^{[2
]}

Cipriano, Michael ^{[7
]}

Mancuso, Joel ^{[8
]}

Tu, Hank ^{[3
]}

Salamov, Asaf ^{[3
]}

Lindquist, Erika ^{[3
]}

Shapiro, Harris ^{[3
]}

Lucas, Susan ^{[3
]}

Grigoriev, Igor V. ^{[3
]}

Cande, W. Zacheus ^{[1
]}

Fulton, Chandler ^{[9
]}

Rokhsar, Daniel S. ^{[1
,3
]}

Dawson, Scott C. ^{[7
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Ctr Integrat Genom, Berkeley, CA 94720 USA

[3] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA

[4] Univ Lancaster, Div Biomed & Life Sci, Sch Hlth & Med, Lancaster LA1 4YQ, England

[5] Univ Alberta, Dept Cell Biol, Edmonton, AB, Canada

[6] Univ Cambridge, Dept Pathol, Cambridge CB2 1QT, England

[7] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA

[8] Gatan Inc, Pleasanton, CA 94588 USA

[9] Brandeis Univ, Dept Biol, Waltham, MA 02454 USA

来源：

CELL | 2010年 / 140卷 / 05期

基金：

英国惠康基金;

关键词：

FATTY-ACID SYNTHESIS; GENE-EXPRESSION; CELL; HYDROGENASE; TRICHOMONAS; EVOLUTION; GIARDIA; ORIGIN; PROTEINS; REVEALS;

D O I：

10.1016/j.cell.2010.01.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoebo-flagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

引用

页码：631 / 642

页数：12

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