Electroporation of nucleic acids into prokaryotic and eukaryotic cells by square wave pulses

被引：9

作者：

Hanze, J ^{[1
]}

Fischer, L

Koenen, M

Worgall, S

Rascher, W

机构：

[1] Univ Giessen, Dept Pediat, D-35390 Giessen, Germany

[2] Biotechnol Res Grp, Heidelberg, Germany

[3] Max Planck Inst Med Res, Heidelberg, Germany

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 02期

关键词：

D O I：

10.1023/A:1008800903452

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel electroporator is presented that generates adjustable square wave pulses with a maximal pulse strength of 2500 V/100 A and a pulse length between 0.1 and 15 ms and that efficiently transfects prokaryotic and eukaryotic cells. Various factors influencing DNA transfer into tissue culture cells, embryonic stem cells and E. coli K12 cells were optimized and the respective electrical pulse profiles monitored.

引用

页码：159 / 163

页数：5

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