Role of MetR and PurR in the activation of glyA by CsgD in Escherichia coli K-12

被引：5

作者：

Chirwa, NT

Herrington, MB

机构：

[1] Concordia Univ, Dept Biol, Montreal, PQ H4B 1R6, Canada

[2] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H4B 1R6, Canada

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2004年 / 50卷 / 09期

关键词：

curli; regulation; serine hydroxymethyltransferase; Hmp; one-carbon; dihydrofolate reductase; MetR; PurR;

D O I：

10.1139/W04-049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The csgD gene of Escherichia coli is required for the expression of curli fibres, surface fibres that are important for biofilm formation and infection. Previously, we demonstrated that expression of CsgD from a multicopy plasmid increased expression of the glyA gene, which codes for serine hydroxymethyltransferase. We show here that this activation requires the participation of both known regulatory proteins, MetR and PurR. The adjacent divergently transcribed gene hmp was weakly induced by CsgD, but its induction did not require MetR or PurR. The effect of CsgD on the expression of several pur and met genes was also tested.

引用

页码：683 / 690

页数：8

共 49 条

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[42] PLASMINOGEN, ABSORBED BY ESCHERICHIA-COLI EXPRESSING CURLI OR BY SALMONELLA-ENTERITIDIS EXPRESSING THIN AGGREGATIVE FIMBRIAE, CAN BE ACTIVATED BY SIMULTANEOUSLY CAPTURED TISSUE-TYPE PLASMINOGEN-ACTIVATOR (T-PA) [J].