Identification of new early auxin markers in tobacco by mRNA differential display

被引：28

作者：

Roux, C ^{[1
]}

Bilang, J ^{[1
]}

Theunissen, BH ^{[1
]}

Perrot-Rechenmann, C ^{[1
]}

机构：

[1] CNRS, Inst Sci Vegetales, F-91198 Gif Sur Yvette, France

来源：

PLANT MOLECULAR BIOLOGY | 1998年 / 37卷 / 02期

关键词：

auxin; differential display; tobacco seedlings; auxin-regulated gene expression;

D O I：

10.1023/A:1005906316880

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Differential display of mRNA has been improved by developing a two-step PCR amplification procedure. The modified differential display has been applied to identify early alterations of mRNA expression in response to auxin treatment of tobacco seedlings. This approach has led to the isolation of four fragments corresponding to auxin-up-regulated mRNAs. One, named GO15-13, shows significant homology with the 3' end of the coding region of the soybean SAUR X10A [10]. The three other fragments present no homology with sequences available in the databases and constitute potential new early auxin markers.

引用

页码：385 / 389

页数：5

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