Electrocatalytic properties of Acetobacter aceti cells immobilized on electrodes for the quinone-mediated oxidation of ethanol

被引:33
作者
Ikeda, T [1 ]
Kato, K
Maeda, M
Tatsumi, H
Kano, K
Matsushita, K
机构
[1] Kyoto Univ, Fac Agr, Dept Agr Chem, Sakyo Ku, Kyoto 606, Japan
[2] Yamaguchi Univ, Fac Agr, Dept Biol Chem, Yamaguchi 753, Japan
来源
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 1997年 / 430卷 / 1-2期
关键词
electrocatalytic oxidation; ethanol; quinone; bacterial cells; biosensors;
D O I
10.1016/S0022-0728(97)00164-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Carbon paste electrodes on which intact cells of Acetobacter aceti (IFO3284) are immobilized behind a dialysis membrane produce a catalytic current for the oxidation of ethanol in the presence of 2-methyl-5,6-dimethoxy benzoquinone (Q(0)) Analysis of the catalytic current reveals that the catalytic activity of the bacterial cells is due to alcohol dehydrogenase (ADH) existing in the cytoplasmic membranes. The membrane-bound ADH catalyzes the oxidation of ethanol by the use of Q(0) penetrating the membranes, in which Q(0) is reduced by accepting electrons from the enzyme. The reduced form of Q(0), in turn, reaches the electrode and is oxidized there. Thus, Q(0) serves as an electron transfer mediator between the intact cells and the electrode. The mediated bioelectrocatalysis behavior can be described by an equation of the catalytic current derived for an enzyme-based electrocatalysis. Performance of the electrode as an ethanol sensor is compared with that of an electrode modified with purified ADH. An ADH deficient strain and the ADH mutant harboring ADH gene are also examined as biocatalysts in place of A. aceti. (C) 1997 Elsevier Science S.A.
引用
收藏
页码:197 / 204
页数:8
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