First proteomic analysis of Legionella pneumophila based on its developing genome sequence

The first proteomic analysis of the respiratory pathogen Legionella pneumophila ATCC 33152 is presented in this report. Two dimensional get electrophoresis of total cell extracts was carried out. In total, 130 protein spots were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MS) or by quadruple time-of-flight tandem MS, including proteins correlated with virulence. For the first time. proteins of L. pneumophila were identified using mass spectrometric methods and mapped on a two-dimensional gel: this will be of considerable use for comparison of protein expression profiles of L. pneumophila wild-type and knock-out mutant strains and of L. pneumophila grown under different conditions. (C) 2004 Elsevier SAS. All rights reserved.