Development of an amperometric biosensor based on acetylcholine esterase covalently bound to a new support material

被引：50

作者：

Khayyami, M

Pita, MTP

Garcia, NP

Johansson, G

Danielsson, B

Larsson, PO

机构：

[1] Univ Lund, Ctr Chem & Chem Engn, Dept Pure & Appl Biochem, S-22100 Lund, Sweden

[2] TMS CHEM AB, S-22370 Lund, Sweden

[3] Univ Lund, Ctr Chem & Chem Engn, Dept Analyt Chem, S-22100 Lund, Sweden

来源：

TALANTA | 1998年 / 45卷 / 03期

关键词：

RVC-electrode; biosensor; acetylcholine esterase; acetylthiocholine; amperometric; thiocholine; mediator; superporous agarose gel;

D O I：

10.1016/S0039-9140(97)00182-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new type of amperometric biosensor based on immobilised acetylcholine esterase was designed and constructed. The enzyme was immobilised on a flow-through working electrode, which was prepared from reticulated vitreous carbon (RVC) or from a composite material consisting of RVC and superporous agarose. The sensor was operated in FIA mode using acetylthiocholine as a substrate. The sensor responded to inhibitors such as paraoxon-10(-9) mol was detected by the sensor in a non-optimised configuration. The practical lifetime of the sensor was at least 1 month. (C) 1998 Elsevier Science B.V.

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收藏

页码：557 / 563

页数：7

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