Proteomic Analysis of Human Osteoblastic Cells: Relevant Proteins and Functional Categories for Differentiation

被引:22
作者
Alves, Rodrigo D. A. M. [1 ]
Eijken, Marco [1 ]
Swagemakers, Sigrid [2 ]
Chiba, H. [4 ]
Titulaer, Mark K. [3 ]
Burgers, Peter C. [3 ]
Luider, Theo M. [3 ]
van Leeuwen, Johannes P. T. M. [1 ]
机构
[1] Erasmus MC, Dept Internal Med, NL-3000 CA Rotterdam, Netherlands
[2] Erasmus MC, Dept Bioinformat, NL-3000 CA Rotterdam, Netherlands
[3] Erasmus MC, Dept Neurol, NL-3000 CA Rotterdam, Netherlands
[4] Sapporo Med Coll, Dept Pathol, Sapporo, Hokkaido 060, Japan
关键词
osteoblast; differentiation; proteomics; MALDI-FT-ICR-MS; INCREASES OSTEOCLAST FORMATION; MESENCHYMAL STEM-CELLS; MASS-SPECTROMETRY; MATRIX VESICLES; ANNEXIN-II; BONE; GROWTH; GENE; PROLIFERATION; EXPRESSION;
D O I
10.1021/pr100400d
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Osteoblasts are the bone forming cells, capable of secreting an extracellular matrix with mineralization potential. The exact mechanism by which osteoblasts differentiate and form a mineralized extracellular matrix is presently not fully understood. To increase our knowledge about this process, we conducted proteomics analysis in human immortalized preosteoblasts (SV-HFO) able to differentiate and mineralize. We identified 381 proteins expressed during the time course of osteoblast differentiation. Gene ontology analysis revealed an overrepresentation of protein categories established as important players for osteoblast differentiation, bone formation, and mineralization such as pyrophosphatases. Proteins involved in antigen presentation, energy metabolism and cytoskeleton rearrangement constitute other overrepresented processes, whose function, albeit interesting, is not fully understood in the context of osteoblast differentiation and bone formation. Correlation analysis, based on quantitative data, revealed a biphasic osteoblast differentiation, encompassing a premineralization and a mineralization period. Identified differentially expressed proteins between mineralized and nonmineralized cells include cytoskeleton (e.g., CCT2, PLEC1, and FLNA) and extracellular matrix constituents (FN1, ANXA2, and LGALS1) among others. FT-ICR-MS data obtained for FN1, ANXA2, and LMNA shows a specific regulation of these proteins during the different phases of osteoblast differentiation. Taken together, this study increases our understanding of the proteomics changes that accompany osteoblast differentiation and may permit the discovery of novel modulators of bone formation.
引用
收藏
页码:4688 / 4700
页数:13
相关论文
共 65 条
[1]   The role of matrix vesicles in growth plate development and biomineralization [J].
Anderson, HC ;
Garimella, R ;
Tague, SE .
FRONTIERS IN BIOSCIENCE-LANDMARK, 2005, 10 :822-837
[2]  
ANDERSON HC, 1995, CLIN ORTHOP RELAT R, P266
[3]   Proteome and proteomics: New technologies, new concepts, and new words [J].
Anderson, NL ;
Anderson, NG .
ELECTROPHORESIS, 1998, 19 (11) :1853-1861
[4]   Regulation of Osteoblast Formation and Function [J].
Aubin J.E. .
Reviews in Endocrine and Metabolic Disorders, 2001, 2 (1) :81-94
[5]   Proteome analysis of matrix vesicles isolated from femurs of chicken embryo [J].
Balcerzak, Marcin ;
Malinowska, Agata ;
Thouverey, Cyril ;
Sekrecka, Anna ;
Dadlez, Michal ;
Buchet, Rene ;
Pikula, Slawomir .
PROTEOMICS, 2008, 8 (01) :192-205
[6]   Comparative evaluation of tandem MS search algorithms using a target-decoy search strategy [J].
Balgley, Brian M. ;
Laudeman, Tom ;
Yang, Li ;
Song, Tao ;
Lee, Cheng S. .
MOLECULAR & CELLULAR PROTEOMICS, 2007, 6 (09) :1599-1608
[7]   Defective collagen crosslinking in bone, but not in ligament or cartilage, in Bruck syndrome: Indications for a bone-specific telopeptide lysyl hydroxylase on chromosome 17 [J].
Bank, RA ;
Robins, SP ;
Wijmenga, C ;
Breslau-Siderius, LJ ;
Bardoel, AFJ ;
Van der Sluijs, HA ;
Pruijs, HEH ;
TeKoppele, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (03) :1054-1058
[8]   Metabolomics applications of FT-ICR mass spectrometry [J].
Brown, SC ;
Kruppa, G ;
Dasseux, JL .
MASS SPECTROMETRY REVIEWS, 2005, 24 (02) :223-231
[9]   Novel markers for the prospective isolation of human MSC [J].
Buehring, Hans-Joerg ;
Battula, Venkata Lokesh ;
Treml, Sabrina ;
Schewe, Bernhard ;
Kanz, Lothar ;
Vogel, Wichard .
HEMATOPOIETIC STEM CELLS VI, 2007, 1106 :262-271
[10]   ACTIN MEMBRANE INTERACTION IN FOCAL ADHESIONS [J].
BURRIDGE, K ;
NUCKOLLS, G ;
OTEY, C ;
PAVALKO, F ;
SIMON, K ;
TURNER, C .
CELL DIFFERENTIATION AND DEVELOPMENT, 1990, 32 (03) :337-342