Fast protocols for the 5S rDNA and ITS-2 based identification of Oenococcus oeni

被引：15

作者：

Hirschhäuser, S ^{[1
]}

Fröhlich, J ^{[1
]}

Gneipel, A ^{[1
]}

Schönig, I ^{[1
]}

König, H ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Microbiol & Wine Res, D-55128 Mainz, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 244卷 / 01期

关键词：

Oenococcus oeni; internal transcribed spacer; 5S rDNA; PCR; fluorescence in situ hybridization;

D O I：

10.1016/j.femsle.2005.01.033

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To identify specific marker sequences for the rapid identification of Oenococcus oeni, we sequenced the 23S-5S internal transcribed spacer (ITS-2) region and the 5S rDNA of five different O.oeni strains and three phylogenetically related lactic acid bacteria (LAB). Comparative analysis revealed 100% identity among the ITS-2 region of the O.oeni strains and remarkable differences in length and sequence compared to related LAB. These results enabled us to develop a primer set for a rapid PCR-identification of O.oeni within three hours. Moreover, the comparison of the 5S rDNA sequences and the highly conserved secondary structure provided the template for the design of three fluorescence-labeled specific oligonucleotides for fluorescence in situ hybridization (FISH). These probes are partial complementary to each other. This feature promotes the accessibility to the target sequence within the ribosome and enhances the fluorescence signal. For the rapid identification of Oenococci both the 5S rRNA gene and the ITS-2 region are useful targets. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：165 / 171

页数：7

共 27 条

[1] Molecular characterization of lactic acid populations associated with wine spoilage [J].

Beneduce, L ;

Spano, G ;

Vernile, A ;

Tarantino, D ;

Massa, S .

JOURNAL OF BASIC MICROBIOLOGY, 2004, 44 (01) :10-16

[2] Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria [J].

Blasco, L ;

Ferrer, S ;

Pardo, I .

FEMS MICROBIOLOGY LETTERS, 2003, 225 (01) :115-123

[3] The Comparative RNA Web (CRW) Site:: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs:: Correction (vol 3, pg 2, 2002) -: art. no. 15 [J].

Cannone, JJ ;

Subramanian, S ;

Schnare, MN ;

Collett, JR ;

D'Souza, LM ;

Du, YS ;

Feng, B ;

Lin, N ;

Madabusi, LV ;

Müller, KM ;

Pande, N ;

Shang, ZD ;

Yu, N ;

Gutell, RR .

BMC BIOINFORMATICS, 2002, 3 (1)

[4] Comparative analysis of the genes encoding 23S-5S rRNA intergenic spacer regions of Lactobacillus casei-related strains [J].

Chen, H ;

Lim, CK ;

Lee, YK ;

Chan, YN .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :471-478

[5] PROPOSAL TO RECLASSIFY LEUCONOSTOC-OENOS AS OENOCOCCUS-OENI [CORRIG] GEN-NOV, COMB-NOV [J].

DICKS, LMT ;

DELLAGLIO, F ;

COLLINS, MD .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1995, 45 (02) :395-397

[6] TAXONOMY OF LEUCONOSTOC SPECIES, PARTICULARLY LEUCONOSTOC-OENOS, AS REVEALED BY NUMERICAL-ANALYSIS OF TOTAL SOLUBLE CELL PROTEIN-PATTERNS, DNA-BASE COMPOSITIONS, AND DNA-DNA HYBRIDIZATIONS [J].

DICKS, LMT ;

VANVUUREN, HJJ ;

DELLAGLIO, F .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1990, 40 (01) :83-91

[7] 5S-RNA SECONDARY STRUCTURE [J].

FOX, GE ;

WOESE, CR .

NATURE, 1975, 256 (5517) :505-507

[8] ARCHITECTURE OF 5S RIBOSOMAL-RNA AND ITS RELATION TO FUNCTION [J].

FOX, GE ;

WOESE, CR .

JOURNAL OF MOLECULAR EVOLUTION, 1975, 6 (01) :61-76

[9]

Fröhlich J, 1999, SYST APPL MICROBIOL, V22, P249, DOI 10.1016/S0723-2020(99)80072-1

[10]

FROHLICH J, 2002, LALLERMAND TECHNICAL, V10, P33

← 1 2 3 →