Immunohistochemical study of vasoactive intestinal peptide (VIP) enteric neurons in diabetic rats supplemented with L-glutamine

被引：25

作者：

Belato Alves, Eder Paulo ^{[1
]}

Pereira Alves, Angela Maria ^{[1
]}

Fernandes Pereira, Renata Virginia ^{[1
]}

de Miranda Neto, Marcilio Hubner ^{[1
]}

Zanoni, Jacqueline Nelisis ^{[1
]}

机构：

[1] Univ Estadual Maringa, Dept Morphophysiol Sci, Lab Enter Neurons, BR-87020900 Maringa, Parana, Brazil

来源：

NUTRITIONAL NEUROSCIENCE | 2010年 / 13卷 / 01期

关键词：

diabetes mellitus; enteric neurons; L-glutamine; vasoactive intestinal peptide; V MYENTERIC NEURONS; OXIDATIVE STRESS; SUBMUCOUS PLEXUS; VITAMIN-E; MYOSIN-V; MORPHOQUANTITATIVE ASPECTS; AUTONOMIC NEUROPATHY; LIPID-PEROXIDATION; ERGIC NEURONS; MELLITUS;

D O I：

10.1179/147683010X12611460763841

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The purpose of this work was to study the area of the varicosities of nerve fibers of myenteric neurons immunoreactive to vasoactive intestinal peptide (VIP-IR) and of the cell bodies of VIP-IR submucosal neurons of the jejunum of diabetic rats supplemented with 2% L-glutamine. Twenty male rats were divided into the following groups: normoglycemic (N), normoglycemic supplemented with L-glutamine (NG), diabetic (D) and diabetic supplemented with L-glutamine (DG). Whole-mounts of the muscle tunica and the submucosal layer were subjected to the immunohistochemical technique for neurotransmitter VIP identification. Morphometric analyses were carried out in 500 VIP-IR cell bodies of submucosal neurons and 2000 VIP-IR varicosities from each group. L-Glutamine supplementation to the normoglycemic animals caused an increase in the areas of the cell bodies (8.49%) and varicosities (21.3%) relative to the controls (P < 0.05). On the other hand, there was a decrease in the areas of the cell bodies (4.55%) and varicosities (28.9%) of group DG compared to those of group D (P < 0.05). It is concluded that L-glutamine supplementation was positive both to normoglycemic and diabetic animals.

引用

页码：43 / 51

页数：9

共 67 条

[1]

AARON I, 1999, AM J MED, V107, P17

[2] Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins [J].