A simple protocol of DNA sequencing with 10% formamide for dissolving G/C compression

被引：2

作者：

Kang, KR ^{[1
]}

Kim, YW ^{[1
]}

机构：

[1] Gyeongsang Natl Univ, Coll Med, Dept Biochem, Chinju 660280, South Korea

来源：

EXPERIMENTAL AND MOLECULAR MEDICINE | 1997年 / 29卷 / 04期

关键词：

formamide; DNA sequencing; T7; polymerase;

D O I：

10.1038/emm.1997.36

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Formamide has been widely used in urea/polyacrylamide gel to solve the compression problems that are occasionally found during the DNA sequencing of G/C rich regions. In this study, however, 10% formamide was added in annealing solution in stead of adding to the gel. The compressions were unfolded efficiently with a more rapid annealing reaction on ice in the presence of 10% formamide.

引用

页码：235 / 237

页数：3

共 9 条

[1]

BEYER HS, 1991, BIOTECHNIQUES, V11, P746

[2] FORMAMIDE DENATURATION OF CHROMOSOMAL DNA FOR IN SITU HYBRIDIZATION AND C-BAND PREPARATION IN GUINEA-PIG, CAVIA-PORCELLUS [J].

HUBBELL, HR ;

SAHASRABUDDHE, CG ;

HSU, TC .

EXPERIMENTAL CELL RESEARCH, 1976, 102 (02) :385-393

[3]

Kim YW, 1996, ONCOGENE, V13, P2275

[4] STRUCTURE-INDEPENDENT NUCLEOTIDE-SEQUENCE ANALYSIS [J].

MILLS, DR ;

KRAMER, FR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (05) :2232-2235

[5] HIGH-THROUGHPUT RNASE PROTECTION ASSAY [J].

MIRONOV, VN ;

VANMONTAGU, M ;

INZE, D .

NUCLEIC ACIDS RESEARCH, 1995, 23 (16) :3359-3360

[6] IMPROVEMENT OF THE DIDEOXY CHAIN TERMINATION METHOD OF DNA SEQUENCING BY USE OF DEOXY-7-DEAZAGUANOSINE TRIPHOSPHATE IN PLACE OF DGTP [J].

MIZUSAWA, S ;

NISHIMURA, S ;

SEELA, F .

NUCLEIC ACIDS RESEARCH, 1986, 14 (03) :1319-1324

[7] USE OF SODIUM TRICHLOROACETATE AND MUNG BEAN NUCLEASE TO INCREASE SENSITIVITY AND PRECISION DURING TRANSCRIPT MAPPING [J].

MURRAY, MG .

ANALYTICAL BIOCHEMISTRY, 1986, 158 (01) :165-170

[8] FORMAMIDE MODIFIED POLYACRYLAMIDE GELS FOR DNA SEQUENCING BY CAPILLARY GEL-ELECTROPHORESIS [J].

ROCHELEAU, MJ ;

GREY, RJ ;

DA, YC ;

HARKE, HR ;

DOVICHI, NJ .

ELECTROPHORESIS, 1992, 13 (08) :484-486

[9] USE OF THIN ACRYLAMIDE GELS FOR DNA SEQUENCING [J].

SANGER, F ;

COULSON, AR .

FEBS LETTERS, 1978, 87 (01) :107-110

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