Efficient cell migration requires global chromatin condensation

被引：105

作者：

Gerlitz, Gabi ^{[1
]}

Bustin, Michael ^{[1
]}

机构：

[1] NCI, Prot Sect, Lab Metab, US Natl Inst Hlth, Bethesda, MD 20892 USA

来源：

JOURNAL OF CELL SCIENCE | 2010年 / 123卷 / 13期

基金：

美国国家卫生研究院;

关键词：

Cell migration; Chromatin; Heterochromatin; HMGs; Histone H1; HISTONE DEACETYLASE INHIBITORS; GENE-EXPRESSION; CHROMOSOMAL-PROTEINS; NUCLEAR-ENVELOPE; DYNAMIC INTERACTIONS; DOMAIN PROTEINS; HDAC INHIBITORS; HEPATOMA-CELLS; TRICHOSTATIN-A; LINKER HISTONE;

D O I：

10.1242/jcs.058271

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Cell migration is a fundamental process that is necessary for the development and survival of multicellular organisms. Here, we show that cell migration is contingent on global condensation of the chromatin fiber. Induction of directed cell migration by the scratch-wound assay leads to decreased DNaseI sensitivity, alterations in the chromatin binding of architectural proteins and elevated levels of H4K20me1, H3K27me3 and methylated DNA. All these global changes are indicative of increased chromatin condensation in response to induction of directed cell migration. Conversely, chromatin decondensation inhibited the rate of cell migration, in a transcription-independent manner. We suggest that global chromatin condensation facilitates nuclear movement and reshaping, which are important for cell migration. Our results support a role for the chromatin fiber that is distinct from its known functions in genetic processes.

引用

页码：2207 / 2217

页数：11

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