Physical factors affecting the production of organic solvent-tolerant protease by Pseudomonas aeruginosa strain K

被引：102

作者：

Abd Rahman, RNZ ^{[1
]}

Geok, LP ^{[1
]}

Basri, M ^{[1
]}

Salleh, AB ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Sci & Environm Studies, Dept Biochem & Microbiol, Serdang 43400, Malaysia

来源：

BIORESOURCE TECHNOLOGY | 2005年 / 96卷 / 04期

关键词：

organic solvent-tolerant protease; physical factors; Pseudomonas aeruginosa;

D O I：

10.1016/j.biortech.2004.06.012

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The physical factors affecting the production of an organic solvent-tolerant protease from Pseudomonas aeruginosa strain K was investigated. Growth and protease production were detected from 37 to 45 degreesC with 37 degreesC being the optimum temperature for P. aeruginosa. Maximum enzyme activity was achieved at static conditions with 4.0% (v/v) inoculum. Shifting the culture from stationary to shaking condition decreased the protease production (6.0-10.0% v/v). Extracellular organic solvent-tolerant protease was detected over a broad pH range from 6.0 to 9.0. However, the highest yield of protease was observed at pH 7.0. Neutral media increased the protease production compared to acidic or alkaline media (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：429 / 436

页数：8

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