Serum-free induced neuronal apoptosis-like cell death is independent of caspase activity

被引：22

作者：

Hamabe, W ^{[1
]}

Fukushima, N ^{[1
]}

Yoshida, A ^{[1
]}

Ueda, H ^{[1
]}

机构：

[1] Nagasaki Univ, Sch Pharmaceut Sci, Dept Mol Pharmacol & Neurosci, Nagasaki 8528521, Japan

来源：

MOLECULAR BRAIN RESEARCH | 2000年 / 78卷 / 1-2期

关键词：

caspase-independent; nuclear fragmentation; primary culture;

D O I：

10.1016/S0169-328X(00)00074-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cultured cortical neurons survived in a density-dependent manner under serum-free conditions. Low-density cultured cells died in an aurintricarboxylic acid (ATA)-sensitive manner, which was accompanied with marked chromatin condensation and nuclear fragmentation. These features, characteristic for apoptosis, were not attenuated by DEVD-CHO, a caspase-3-specific inhibitor, or zVAD-FMK, a broad range caspase inhibitor, while zVAD-FMK showed a marked inhibition of camptothecin-induced cell death. Therefore, cortical neurons died in an apoptosis-like and a caspase-independent manner under serum-free conditions. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：186 / 191

页数：6

共 38 条

[1] Selective involvement of caspase-3 in ceramide induced apoptosis in AK-5 tumor cells [J].

Anjum, R ;

Ali, AM ;

Begum, Z ;

Vanaja, J ;

Khar, A .

FEBS LETTERS, 1998, 439 (1-2) :81-84

[2] USE OF CENTRAL NEURONAL CULTURES FOR THE DETECTION OF NEURONOTROPHIC AGENTS [J].

BARBIN, G ;

SELAK, I ;

MANTHORPE, M ;

VARON, S .

NEUROSCIENCE, 1984, 12 (01) :33-43

[3] AURINTRICARBOXYLIC ACID RESCUES PC12 CELLS AND SYMPATHETIC NEURONS FROM CELL-DEATH CAUSED BY NERVE GROWTH-FACTOR DEPRIVATION - CORRELATION WITH SUPPRESSION OF ENDONUCLEASE ACTIVITY [J].

BATISTATOU, A ;

GREENE, LA .

JOURNAL OF CELL BIOLOGY, 1991, 115 (02) :461-471

[4] Caspases: the executioners of apoptosis [J].

Cohen, GM .

BIOCHEMICAL JOURNAL, 1997, 326 :1-16

[5] Proteases to die for [J].

Cryns, V ;

Yuan, JY .

GENES & DEVELOPMENT, 1998, 12 (11) :1551-1570

[6]

DALTOSO R, 1988, J NEUROSCI, V8, P733

[7] Genetic and metabolic status of NGF-deprived sympathetic neurons saved by an inhibitor of ICE family proteases [J].

Deshmukh, M ;

Vasilakos, J ;

Deckwerth, TL ;

Lampe, PA ;

Johnson, EM .

JOURNAL OF CELL BIOLOGY, 1996, 135 (05) :1341-1354

[8] A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD [J].

Enari, M ;

Sakahira, H ;

Yokoyama, H ;

Okawa, K ;

Iwamatsu, A ;

Nagata, S .

NATURE, 1998, 391 (6662) :43-50

[9] Caspase inhibition selectively reduces the apoptotic component of oxygen-glucose deprivation-induced cortical neuronal cell death [J].

Gottron, FJ ;

Ying, HS ;

Choi, DW .

MOLECULAR AND CELLULAR NEUROSCIENCE, 1997, 9 (03) :159-169

[10]

HARTIKKA J, 1988, J NEUROSCI, V8, P2967

← 1 2 3 4 →