Interactions among three distinct CesA proteins essential for cellulose synthesis

被引:558
作者
Taylor, NG
Howells, RM
Huttly, AK
Vickers, K
Turner, SR [1 ]
机构
[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England
[2] Inst Arable Crops Res, Bristol BS41 9AF, Avon, England
关键词
D O I
10.1073/pnas.0337628100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
In a screen to identify novel cellulose deficient mutants, three lines were shown to be allelic and define a novel complementation group, irregular xylem5 (irx5). IRX5 was cloned and encodes a member of the CesA family of cellulose synthase catalytic subunits (AtCesA4). irx5 plants have an identical phenotype to previously described mutations in two other members of this gene family (IRX1 and IRX3). IRX5, IRX3, and IRX1 are coexpressed in exactly the same cells, and all three proteins interact in detergent solubilized extracts, suggesting that three members of this gene family are required for cellulose synthesis in secondary cell walls. The association of IRX1 and IRX3 was reduced to undetectable levels in the absence of IRX5. Consequently, these data suggest that IRX5, IRX3, and IRX1 are all essential components of the cellulose synthesizing complex and the presence of all three subunits is required for the correct assembly of this complex.
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页码:1450 / 1455
页数:6
相关论文
共 24 条
[1]  
[Anonymous], 1988, Antibodies: A Laboratory Manual
[2]   Molecular analysis of cellulose biosynthesis in Arabidopsis [J].
Arioli, T ;
Peng, LC ;
Betzner, AS ;
Burn, J ;
Wittke, W ;
Herth, W ;
Camilleri, C ;
Höfte, H ;
Plazinski, J ;
Birch, R ;
Cork, A ;
Glover, J ;
Redmond, J ;
Williamson, RE .
SCIENCE, 1998, 279 (5351) :717-720
[3]   β-subunit assembly is essential for the correct packing and the stable membrane insertion of the H,K-ATPase α-subunit [J].
Beggah, AT ;
Béguin, P ;
Bamberg, K ;
Sachs, G ;
Geering, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (12) :8217-8223
[4]   The biosynthesis of cellulose [J].
Brown, RM .
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY, 1996, A33 (10) :1345-1373
[5]   THE PLASMA-MEMBRANE OF ARABIDOPSIS-THALIANA CONTAINS A MERCURY-INSENSITIVE AQUAPORIN THAT IS A HOMOLOG OF THE TONOPLAST WATER CHANNEL PROTEIN TIP [J].
DANIELS, MJ ;
MIRKOV, TE ;
CHRISPEELS, MJ .
PLANT PHYSIOLOGY, 1994, 106 (04) :1325-1333
[6]   Resistance against herbicide isoxaben and cellulose deficiency caused by distinct mutations in same cellulose synthase isoform CESA6 [J].
Desprez, T ;
Vernhettes, S ;
Fagard, M ;
Refrégier, G ;
Desnos, T ;
Aletti, E ;
Py, N ;
Pelletier, S ;
Höfte, H .
PLANT PHYSIOLOGY, 2002, 128 (02) :482-490
[7]   A SIMPLE AND RAPID METHOD FOR THE PREPARATION OF PLANT GENOMIC DNA FOR PCR ANALYSIS [J].
EDWARDS, K ;
JOHNSTONE, C ;
THOMPSON, C .
NUCLEIC ACIDS RESEARCH, 1991, 19 (06) :1349-1349
[8]   PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and dark-grown hypocotyls of arabidopsis [J].
Fagard, M ;
Desnos, T ;
Desprez, T ;
Goubet, F ;
Refregier, G ;
Mouille, G ;
McCann, M ;
Rayon, C ;
Vernhettes, S ;
Höfte, H .
PLANT CELL, 2000, 12 (12) :2409-2423
[9]  
GEERING K, 1997, MOL B INT U, P173
[10]   Structure of cellulose-deficient secondary cell walls from the irx3 mutant of Arabidopsis thaliana [J].
Ha, MA ;
MacKinnon, IM ;
Sturcová, A ;
Apperley, DC ;
McCann, MC ;
Turner, SR ;
Jarvis, MC .
PHYTOCHEMISTRY, 2002, 61 (01) :7-14