Complete sequences of the S-genes, Sd- and Sh-RNase cDNA in apple

被引:46
作者
Kitahara, K
Soejima, J
Komatsu, H
Fukui, H
Matsumoto, S [1 ]
机构
[1] Gifu Univ, Fac Educ, Dept Biol, Gifu 5011193, Japan
[2] Gifu Univ, Sch Agr Sci, United Grad Sch Agr Sci, Gifu 5011193, Japan
[3] Natl Inst Fruit Tree Sci, Apple Res Ctr, Morioka, Iwate 0200123, Japan
[4] Nagano Fruit Tree Expt Stn, Nagano 3820072, Japan
[5] Gifu Univ, Fac Agr, Lab Hort, Gifu 5011193, Japan
关键词
Malus x domestica; S-allele; PCR; pistil; leaf;
D O I
10.21273/HORTSCI.35.4.712
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
The S-locus genes in the pistil (S-RNases) were cloned from the apple (Malus xdomestica Borkh.) cultivar Akane (S-genotype SdSh from pollination analysis). The Sd- and Sh-RNase corresponded to S7- and S24-RNase, which have been cloned from 'Idared' and 'Braeburn', respectively. Sh-RNase was very similar to Sf- and Sg-RNases at the deduced amino acid-sequence levels (93%). We developed an S-allele specific polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis method for distinguishing the Sh from Sf and Sg, and the Sh-alleles of 'Akane', 'Touhoku 2'. 'Vista Bella', and 'Worcester Pearmain' were identified. We also identified the S-allele genotypes of 16 apple cultivars.
引用
收藏
页码:712 / 715
页数:4
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