Complete sequences of the S-genes, Sd- and Sh-RNase cDNA in apple

被引：46

作者：

Kitahara, K

Soejima, J

Komatsu, H

Fukui, H

Matsumoto, S ^{[1
]}

机构：

[1] Gifu Univ, Fac Educ, Dept Biol, Gifu 5011193, Japan

[2] Gifu Univ, Sch Agr Sci, United Grad Sch Agr Sci, Gifu 5011193, Japan

[3] Natl Inst Fruit Tree Sci, Apple Res Ctr, Morioka, Iwate 0200123, Japan

[4] Nagano Fruit Tree Expt Stn, Nagano 3820072, Japan

[5] Gifu Univ, Fac Agr, Lab Hort, Gifu 5011193, Japan

来源：

HORTSCIENCE | 2000年 / 35卷 / 04期

关键词：

Malus x domestica; S-allele; PCR; pistil; leaf;

D O I：

10.21273/HORTSCI.35.4.712

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

The S-locus genes in the pistil (S-RNases) were cloned from the apple (Malus xdomestica Borkh.) cultivar Akane (S-genotype SdSh from pollination analysis). The Sd- and Sh-RNase corresponded to S7- and S24-RNase, which have been cloned from 'Idared' and 'Braeburn', respectively. Sh-RNase was very similar to Sf- and Sg-RNases at the deduced amino acid-sequence levels (93%). We developed an S-allele specific polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis method for distinguishing the Sh from Sf and Sg, and the Sh-alleles of 'Akane', 'Touhoku 2'. 'Vista Bella', and 'Worcester Pearmain' were identified. We also identified the S-allele genotypes of 16 apple cultivars.

引用

页码：712 / 715

页数：4

共 12 条

[1] CDNA CLONING AND MOLECULAR ANALYSIS OF 2 SELF-INCOMPATIBILITY ALLELES FROM APPLE [J].