Protein deformation of lipid hybrid bilayer membranes studied by sum frequency generation vibrational spectroscopy

被引：53

作者：

Doyle, AW

Fick, J

Himmelhaus, M

Eck, W

Graziani, I

Prudovsky, I

Grunze, M

Maciag, T

Neivandt, DJ ^{[1
]}

机构：

[1] Univ Maine, Dept Biol & Chem Engn, Orono, ME 04469 USA

[2] Heidelberg Univ, Dept Appl Phys Chem, D-69120 Heidelberg, Germany

[3] Maine Med Ctr, Res Inst, Ctr Mol Med, Scarborough, ME 04704 USA

[4] Jackson Lab, Inst Mol Biophys, Bar Harbor, ME 04609 USA

来源：

LANGMUIR | 2004年 / 20卷 / 21期

关键词：

D O I：

10.1021/la0484220

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Structural deformations of lipid hybrid bilayer membranes induced by signal peptideless (SPL) proteins have been studied for the first time using the inherently surface specific nonlinear optical technique of sum frequency generation vibrational spectroscopy. Specifically, deformations of 1,2-distearoylphosphatidylglycerol (DSPG) membranes induced by interaction with FGF-1, a SPL protein which is released as a function of cellular stress through a nonclassical pathway, have been investigated. FGF-1 was found to induce lipid alkyl chain deformations in previously highly ordered DSPG membranes at the extremely low concentration of 1 nM at 60degreesC. The deformation process was shown to exhibit a degree of reversibility upon removal of the protein by rinsing with buffer solution.

引用

页码：8961 / 8965

页数：5

共 32 条

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