Functionalizing surfaces with nickel ions for the grafting of proteins

被引:26
作者
Du Roure, O
Debiemme-Chouvy, C
Malthête, J
Silberzan, P
机构
[1] Inst Curie, Sect Rech, UMR CNRS 168, F-75231 Paris 05, France
[2] Univ Versailles, Inst Laboisier, IREM, UMR CNRS, F-78000 Versailles, France
关键词
D O I
10.1021/la020636z
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We study the grafting of newly synthesized nickel-chelating molecules on gold surfaces via their thiol moiety. These functionalized surfaces can be used as templates to anchor recombinant proteins engineered to bear a sequence of six histidines (His-tag). To tune the nickel concentration on the surface and ultimately the protein density, we have grafted mixed monolayers in which these nickel-chelating molecules are diluted by molecules that have a similar structure but are unable to fix Ni2+ ions. We have used different complementary techniques such as contact angle measurements, ellipsometry, and high-resolution X-ray photoelectron spectroscopy (XPS) to characterize the structures of these monolayers. XPS analyses were also performed to quantify the amount of nickel fixed on the surfaces. We find that the surface composition mirrors well the relative concentrations in the grafting solution. The robustness of the complexation of the Ni2+ ions was probed by exchange and competition experiments. His-tagged protein fragments could be strongly anchored on these surfaces as probed by atomic force microscopy experiments.
引用
收藏
页码:4138 / 4143
页数:6
相关论文
共 30 条
[1]   SPONTANEOUSLY ORGANIZED MOLECULAR ASSEMBLIES .1. FORMATION, DYNAMICS, AND PHYSICAL-PROPERTIES OF NORMAL-ALKANOIC ACIDS ADSORBED FROM SOLUTION ON AN OXIDIZED ALUMINUM SURFACE [J].
ALLARA, DL ;
NUZZO, RG .
LANGMUIR, 1985, 1 (01) :45-52
[2]   FORMATION OF MONOLAYERS BY THE COADSORPTION OF THIOLS ON GOLD - VARIATION IN THE HEAD GROUP, TAIL GROUP, AND SOLVENT [J].
BAIN, CD ;
EVALL, J ;
WHITESIDES, GM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (18) :7155-7164
[3]   A STUDY BY CONTACT-ANGLE OF THE ACID-BASE BEHAVIOR OF MONOLAYERS CONTAINING OMEGA-MERCAPTOCARBOXYLIC ACIDS ADSORBED ON GOLD - AN EXAMPLE OF REACTIVE SPREADING [J].
BAIN, CD ;
WHITESIDES, GM .
LANGMUIR, 1989, 5 (06) :1370-1378
[4]  
Beamson G., 1992, HIGH RESOLUTION XPS, P196
[5]   Voltammetric and XPS investigations of nickel hydroxide electrochemically dispersed on gold surface electrodes [J].
Casella, IG ;
Guascito, MR ;
Sannazzaro, MG .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 462 (02) :202-210
[6]   Wettability of porous surfaces. [J].
Cassie, ABD ;
Baxter, S .
TRANSACTIONS OF THE FARADAY SOCIETY, 1944, 40 :0546-0550
[7]  
DUROURE O, IN PRESS
[8]  
FERACCI H, COMMUNICATION
[9]   How strong is a covalent bond? [J].
Grandbois, M ;
Beyer, M ;
Rief, M ;
Clausen-Schaumann, H ;
Gaub, HE .
SCIENCE, 1999, 283 (5408) :1727-1730
[10]   SYNTHESIS AND APPLICATIONS OF A NEW POLY(ETHYLENE GLYCOL) DERIVATIVE FOR THE CROSS-LINKING OF AMINES WITH THIOLS [J].
HASELGRUBLER, T ;
AMERSTORFER, A ;
SCHINDLER, H ;
GRUBER, HJ .
BIOCONJUGATE CHEMISTRY, 1995, 6 (03) :242-248