Methods for the estimation of binding constants by capillary electrophoresis

被引:149
作者
Rundlett, KL [1 ]
Armstrong, DW [1 ]
机构
[1] Univ Missouri, Dept Chem, Rolla, MO 65401 USA
关键词
equilibrium constants; molecular association; linear plotting; nonlinear regression; errors;
D O I
10.1002/elps.1150181210
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Capillary electrophoresis (CE) has developed into a particularly effective means to determine apparent equilibrium constants for molecular association in solution (e.g., to micelles, cyclodextrins. antibiotics, proteins, RNA, DNA, etc.). The various experimental, graphical and mathematical approaches for determining association constants are reviewed. In CE, association constants can be calculated because there is a relationship between substrate concentration and the measured electrophoretic mobility of the solute. Most of the approaches for obtaining association constants by CE are conceptually and mathematically related to one another, Likewise, they are analogous to many spectroscopic techniques that are used for obtaining association constants. The advantages, limitations and proper use of the various CE approaches are examined.
引用
收藏
页码:2194 / 2202
页数:9
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