Expression of IL-4, Cε RNA, and Iε RNA in the nasal mucosa of patients with seasonal rhinitis:: Effect of topical corticosteroids

被引:62
作者
Cameron, LA
Durham, SR
Jacobson, MR
Masuyama, K
Juliusson, S
Gould, HJ
Lowhagen, O
Minshall, EM
Hamid, OA
机构
[1] McGill Univ, Meakins Christie Labs, Quebec City, PQ H2X 2P2, Canada
[2] Royal Brompton Hosp, Natl Heart & Lung Inst, London SW3 6LY, England
[3] Univ London Kings Coll, Randall Inst, London WC2R 2LS, England
[4] Sahlgrenska Hosp, Allergy Clin, Gothenburg, Sweden
基金
英国医学研究理事会;
关键词
IgE; C epsilon; I epsilon; IL-4; allergic rhinitis; topical corticosteroids;
D O I
10.1016/S0091-6749(98)70244-1
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: Nasal allergen provocation has demonstrated that allergen-induced rhinitis is associated with an increase in local IL-4 mRNA and IgE heavy chain (C epsilon) and IgE heavy chain promoter (I epsilon) RNA and that pretreatment with topical glucocorticosteroids inhibits the increase in these transcripts. Objective; This study was undertaken ta determine whether observations made after acute allergen provocation can be extended to the case of chronic exposure experienced during the pollen season. Methods: Biopsy specimens were obtained from the inferior turbinate of 33 pollen-sensitive subjects with allergic rhinitis before and during pollen season. Patients were randomized in a double-blind fashion and treated with either topical steroids (200 mu g fluticasone propionate twice daily; n = 16) or matched placebo nasal sprag (n = 17) before the pollen season. Alkaline phospatase anti-alkaline phosphatase immunocytochemistry was used to identify B cells (CD20(+)), and in situ hybridization was used to detect IL-4, C epsilon and I epsilon RNA(+) cells. Results: Baseline examination revealed IL-4 and C epsilon RNA but virtually no I epsilon RNA(+) cells in the nasal mucosa. Analysis revealed a significant difference in the expression of C epsilon and I epsilon RNA(+) cells (p < 0.001). Biopsy specimens taken after antigen exposure exhibited highly significant increases in placebo-treated (p < 0.001) but not steroid-treated patients. In both groups, the number of CD20(+) cells was unchanged when preexposure and postexposure biopsy specimens were compared. Conclusions: These results show strong support for the hypothesis that IgE class switching occurs locally within the nasal mucosa of subjects with seasonal allergic rhinitis and that this response can be inhibited through strategies directed against local IgE production.
引用
收藏
页码:330 / 336
页数:7
相关论文
共 35 条
[1]  
ABEHSIRAAMAR O, 1992, J IMMUNOL, V148, P3820
[2]   THE INFLUX OF INFLAMMATORY CELLS INTO NASAL WASHINGS DURING THE LATE RESPONSE TO ANTIGEN CHALLENGE - EFFECT OF SYSTEMIC STEROID PRETREATMENT [J].
BASCOM, R ;
PIPKORN, U ;
LICHTENSTEIN, LM ;
NACLERIO, RM .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1988, 138 (02) :406-412
[3]   Germinal center formation and local immunoglobulin E (IgE) production in the lung after an airway antigenic challenge [J].
Chvatchko, Y ;
KoscoVilbois, MH ;
Herren, S ;
Lefort, J ;
Bonnefoy, JY .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 184 (06) :2353-2360
[4]  
DELPRETE G, 1988, J IMMUNOL, V140, P4193
[5]   Local IgE production in nasal allergy [J].
Durham, SR ;
Gould, HJ ;
Hamid, QA .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1997, 113 (1-3) :128-130
[6]   Expression of epsilon germ-line gene transcripts and mRNA for the epsilon heavy chain of IgE in nasal B cells and the effects of topical corticosteroid [J].
Durham, SR ;
Gould, HJ ;
Thienes, CP ;
Jacobson, MR ;
Masuyama, K ;
Rak, S ;
Lowhagen, O ;
Schotman, E ;
Cameron, L ;
Hamid, QA .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (11) :2899-2906
[7]  
DURHAM SR, 1992, J IMMUNOL, V148, P2390
[8]   STRUCTURE AND EXPRESSION OF GERMLINE EPSILON-TRANSCRIPTS IN HUMAN B-CELLS INDUCED BY INTERLEUKIN-4 TO SWITCH TO IGE PRODUCTION [J].
GAUCHAT, JF ;
LEBMAN, DA ;
COFFMAN, RL ;
GASCAN, H ;
DEVRIES, JE .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (02) :463-473
[9]  
GESSNER A, 1994, INFECT IMMUN, V62, P411
[10]   WHERE DO MUCOSAL MAST-CELLS ACQUIRE IGE [J].
GILLON, J .
IMMUNOLOGY TODAY, 1981, 2 (05) :80-81