Developmental genes have pleiotropic effects on plant morphology and source capacity, eventually impacting on seed protein content and productivity in pea

被引:90
作者
Burstin, Judith [1 ]
Marget, Pascal
Huart, Myriam
Moessner, Annie
Mangin, Brigitte
Duchene, Christiane
Desprez, Bruno
Munier-Jolain, Nathalie
Duc, Gerard
机构
[1] INRA, UR102 Genet & Ecophysiol Grain Legumes, F-21110 Bretenieres, France
[2] Bioplant GIE, F-59930 La Chapelle Armentiers, France
[3] Bioplant GIE, Chartainvilliers, France
[4] Biogemma Nickerson, F-28130 Chartainvilliers, France
[5] INRA, Biometrie & Intelligence Artificielle, F-31326 Castanet Tolosan, France
关键词
D O I
10.1104/pp.107.096966
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Increasing pea (Pisum sativum) seed nutritional value and particularly seed protein content, while maintaining yield, is an important challenge for further development of this crop. Seed protein content and yield are complex and unstable traits, integrating all the processes occurring during the plant life cycle. During filling, seeds are the main sink to which assimilates are preferentially allocated at the expense of vegetative organs. Nitrogen seed demand is satisfied partly by nitrogen acquired by the roots, but also by nitrogen remobilized from vegetative organs. In this study, we evaluated the respective roles of nitrogen source capacity and sink strength in the genetic variability of seed protein content and yield. We showed in eight genotypes of diverse origins that both the maximal rate of nitrogen accumulation in the seeds and nitrogen source capacity varied among genotypes. Then, to identify the genetic factors responsible for seed protein content and yield variation, we searched for quantitative trait loci (QTL) for seed traits and for indicators of sink strength and source nitrogen capacity. We detected 261 QTL across five environments for all traits measured. Most QTL for seed and plant traits mapped in clusters, raising the possibility of common underlying processes and candidate genes. In most environments, the genes Le and Afila, which control internode length and the switch between leaflets and tendrils, respectively, determined plant nitrogen status. Depending on the environment, these genes were linked to QTL of seed protein content and yield, suggesting that source-sink adjustments depend on growing conditions.
引用
收藏
页码:768 / 781
页数:14
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