dAkt kinase controls follicle cell size during Drosophila oogenesis

被引:23
作者
Cavaliere, V
Donati, A
Hsouna, A
Hsu, T
Gargiulo, G
机构
[1] Dipartimento Biol Evoluzionist Sperimentale, I-40126 Bologna, Italy
[2] Med Univ S Carolina, Dept Pathol & Lab Med, Charleston, SC USA
[3] Med Univ S Carolina, Hollings Canc Ctr, Charleston, SC USA
关键词
cell growth; dAkt; follicle cells; oogenesis; Drosophila;
D O I
10.1002/dvdy.20333
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
The Drosophila Akt (dAkt) serine/threonine kinase is a component of the insulin receptor/PI3K signaling pathway that regulates cell growth. Here, we show that this kinase is expressed during Drosophila oogenesis and is required for egg chamber development. Loss of dAkt function in follicle cells causes a cell-autonomous reduction of cell size while expression of the constitutively active myristylated form of this kinase (dAkt(myr)) causes increased cell size. Accordingly, expression of the antagonist dPTEN in the same follicular domains causes reduced follicle cell size. Perturbations of dAkt function do not affect follicle cell proliferation or cell death. Of interest, expression of dAkt(myr) in the posterior domain of the follicular epithelium causes a delay in the posterior movement of follicular epithelium and dumpless-like egg chambers. It appears that dAkt is required for maintaining the continuity of cell size within the follicular epithelium, which in turn is necessary for its proper morphogenesis. (C) 2005 Wiley-Liss, Inc.
引用
收藏
页码:845 / 854
页数:10
相关论文
共 45 条
[1]   DEVELOPMENTAL REGULATION OF EXPRESSION AND ACTIVITY OF MULTIPLE FORMS OF THE DROSOPHILA RAC PROTEIN-KINASE [J].
ANDJELKOVIC, M ;
JONES, PF ;
GROSSNIKLAUS, U ;
CRON, P ;
SCHIER, AF ;
DICK, M ;
BILBE, G ;
HEMMINGS, BA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (08) :4066-4075
[2]   Role of translocation in the activation and function of protein kinase B [J].
Andjelkovic, M ;
Alessi, DR ;
Meier, R ;
Fernandez, A ;
Lamb, NJC ;
Frech, M ;
Cron, P ;
Cohen, P ;
Lucocq, JM ;
Hemmings, BA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (50) :31515-31524
[3]  
Andrenacci D, 2001, J CELL SCI, V114, P2819
[4]   Autonomous control of cell and organ size by CHICO, a Drosophila homolog of vertebrate IRS1-4 [J].
Böhni, R ;
Riesgo-Escovar, J ;
Oldham, S ;
Brogiolo, W ;
Stocker, H ;
Andruss, BF ;
Beckingham, K ;
Hafen, E .
CELL, 1999, 97 (07) :865-875
[5]   RAF ACTS DOWNSTREAM OF THE EGF RECEPTOR TO DETERMINE DORSOVENTRAL POLARITY DURING DROSOPHILA OOGENESIS [J].
BRAND, AH ;
PERRIMON, N .
GENES & DEVELOPMENT, 1994, 8 (05) :629-639
[6]  
BRAND AH, 1993, DEVELOPMENT, V118, P401
[7]   Cell cycle control of chorion gene amplification [J].
Calvi, BR ;
Lilly, MA ;
Spradling, AC .
GENES & DEVELOPMENT, 1998, 12 (05) :734-744
[8]   The Drosophila insulin receptor is required for normal growth [J].
Chen, C ;
Jack, J ;
Garofalo, RS .
ENDOCRINOLOGY, 1996, 137 (03) :846-856
[9]   Drosophila phosphoinositide-dependent kinase-1 regulates apoptosis and growth via the phosphoinositide 3-kinase-dependent signaling pathway [J].
Cho, KS ;
Lee, JH ;
Kim, S ;
Kim, D ;
Koh, H ;
Lee, J ;
Kim, C ;
Kim, J ;
Chung, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (11) :6144-6149
[10]   ALTERNATIVE SITES FOR ECDYSTEROID PRODUCTION IN INSECTS [J].
DELBECQUE, JP ;
WEIDNER, K ;
HOFFMANN, KH .
INVERTEBRATE REPRODUCTION & DEVELOPMENT, 1990, 18 (1-2) :29-42