Convective flow effects on DNA biosensors

被引：10

作者：

Bishop, J. ^{[1
]}

Blair, S. ^{[1
]}

Chagovetz, A. ^{[1
]}

机构：

[1] Univ Utah, Dept Elect & Comp Engn, Salt Lake City, UT 84112 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 9-10期

基金：

美国国家科学基金会;

关键词：

mass transport; convective flow; recirculation; thermodynamic equilibrium; nucleic acid arrays;

D O I：

10.1016/j.bios.2006.10.020

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We explore theoretic limits of convective flux effects, as a function of species concentrations and sample volume, in biosensing systems containing target species with differing affinities. A finite element method is used to simulate mass transport of DNA through a microfluidic chamber to the sensing surface where hybridization of DNA is modeled using the corresponding kinetic equations. Our results show enhancements due to convection are controlled by the concentration and sample volume of the lower affinity species, while the upper-limit of the enhancements are determined by the concentration and sample volume of the higher affinity species. Additionally, enhancements are controlled by the degree of competition between the two species, which is related to the total fraction of binding sites used. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：2192 / 2198

页数：7

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