Interactions of the ribosome with mRNA and tRNA

被引：34

作者：

Demeshkina, Natalia ^{[1
,2
]}

Jenner, Lasse ^{[1
,3
]}

Yusupova, Gulnara ^{[1
,2
]}

Yusupov, Marat ^{[1
,2
]}

机构：

[1] Inst Genet & Biol Mol & Cellulaire, Dept Biol & Genom Struct, F-67400 Illkirch Graffenstaden, France

[2] CNRS, UMR7104, F-67400 Illkirch Graffenstaden, France

[3] INSERM, U964, F-67400 Illkirch Graffenstaden, France

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 2010年 / 20卷 / 03期

关键词：

CRYSTAL-STRUCTURE; 70S RIBOSOME; ANGSTROM RESOLUTION; HYBRID STATE; SUBUNIT; INSIGHTS; IDENTIFICATION; TRANSLOCATION; MECHANISM;

D O I：

10.1016/j.sbi.2010.03.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent collection of high-resolution crystal structures of the 70S ribosome with mRNA and tRNA substrates enhances our knowledge of protein synthesis principles. A novel network of interactions between the ribosome in the elongation state and mRNA downstream from the A codon suggests that mRNA is stabilized and aligned at the entrance to the decoding center. The X-ray studies clarify how natural modifications of tRNA are involved in the stabilization of the codon-anticodon interactions, prevention of frame-shifting and also expansion of the decoding capacity of tRNAs. In addition, the crystal structures provide the view that tRNA in the A and P sites communicate through a protein rich environment and suggest how these tRNAs are controlled through the intersubunit bridge formed by protein L31.

引用

页码：325 / 332

页数：8

共 38 条

[1] Visualization of the Hybrid State of tRNA Binding Promoted by Spontaneous Ratcheting of the Ribosome [J].