Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin

被引：25

作者：

de Frutos, M

Cifuentes, A

Diez-Masa, JC

机构：

[1] CSIC, Inst Organ Chem, E-28006 Madrid, Spain

[2] CSIC, Inst Ind Fermentat, E-28006 Madrid, Spain

来源：

ELECTROPHORESIS | 2003年 / 24卷 / 04期

关键词：

capillary zone electrophoresis; doping; erythropoietin; glycoprotein;

D O I：

10.1002/elps.200390080

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Different profiles were obtained by capillary zone electrophoresis (CZE) of human erythropoietin (EPO) of recombinant and urinary origin. To unambiguously detect doping by EPO, direct methods able to determine the presence of the drug itself in a physiological fluid are required. Since the host cell line used for EPO production influences its glycosylation, the carbohydrate distribution of natural human EPO may be different from that of recombinant EPO. The different content in sialic acid groups between recombinant and endogenous EPO provide a basis for their distinction by CZE.

引用

收藏

页码：678 / 680

页数：3

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