Determination of transforming growth factor-β2 (TGF-β2) in bovine colostrum samples

被引：47

作者：

Pakkanen, R ^{[1
]}

机构：

[1] Nutricia Fennica Ltd, FIN-20100 Turku, Finland

来源：

JOURNAL OF IMMUNOASSAY | 1998年 / 19卷 / 01期

关键词：

TGF-beta; colostrum; ELISA; growth factor;

D O I：

10.1080/01971529808005469

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor-beta 2 (TGF-beta 2) is the major TGF-beta form in bovine colostrum. A colostrum pool of the five first milkings was made to validate an ELISA specific for human TGF-beta 2 for measure TGF-beta 2 concentration in bovine colostrum samples. According to this test >90% of total TGF-beta 2 (74.5+/-4.4 ng/ml) in colostrum pool was in a latent form that could be activated by acetic acid treatment, whereas the concentration of the active form was only 4.19+/-0.27 ng/ml. Activated colostrum samples of the first milkings of five cows contained 150-1150 ng TGF-beta 2/ml and its concentration declined in correlation (r=0.86) with total protein concentration to 12-71 ng/ml by the fifth milkings. Most of the TGF-beta 2 (94%) was found in the whey fraction of colostrum. The ELISA results were also compared with a TGF-beta 2 bioassay, the fibroblasts migration assay. This assay detected 9.8+/-1.0 ng/ml and 4.4+/-0.7 ng/ml in the activated and non-activated samples of colostrum pool respectively.

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页码：23 / 37

页数：15

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