GUP1 and its close homologue GUP2, encoding multimembrane-spanning proteins involved in active glycerol uptake in Saccharomyces cerevisiae

被引:82
作者
Holst, B
Lunde, C
Lages, F
Oliveira, R
Lucas, C
Kielland-Brandt, MC
机构
[1] Carlsberg Lab, Dept Yeast Genet, DK-2500 Copenhagen, Denmark
[2] Univ Minho, CCA, Dept Biol, P-4709 Braga, Portugal
关键词
D O I
10.1046/j.1365-2958.2000.01968.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many yeast species can utilize glycerol, both as a sole carbon source and as an osmolyte. In Saccharomyces cerevisiae, physiological studies have previously shown the presence of an active uptake system driven by electrogenic proton symport. We have used transposon mutagenesis to isolate mutants affected in the transport of glycerol into the cell. Here we present the identification of YGL084c, encoding a multimembrane-spanning protein, as being essential for proton symport of glycerol into S. cerevisiae. The gene is named GUP1 (glycerol uptake) and, for growth on glycerol, is important as a carbon and energy source. In addition, in strains deficient in glycerol production it also provides osmotic protection by the addition of glycerol. Another open reading frame (ORF), YPL189w, presenting a high degree of homology to YGL084c, similarly appears to be involved in active glycerol uptake in salt-containing glucose-based media in strains deficient in glycerol production. Analogously, this gene is named GUP2. To our knowledge, this is the first report on a gene product involved in active transport of glycerol in yeasts. Mutations with the same phenotypes occurred in two other ORFs of previously unknown function, YDL074c and YPL180w.
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页码:108 / 124
页数:17
相关论文
共 43 条
[1]   GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY [J].
ALBERTYN, J ;
HOHMANN, S ;
THEVELEIN, JM ;
PRIOR, BA .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (06) :4135-4144
[2]  
[Anonymous], METHOD ENZYMOL
[3]   The two isoenzymes for yeast NAD(+)-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation [J].
Ansell, R ;
Granath, K ;
Hohmann, S ;
Thevelein, JM ;
Adler, L .
EMBO JOURNAL, 1997, 16 (09) :2179-2187
[4]   Osmoresponsive proteins and functional assessment strategies in Saccharomyces cerevisiae [J].
Blomberg, A .
ELECTROPHORESIS, 1997, 18 (08) :1429-1440
[5]   MULTIFUNCTIONAL YEAST HIGH-COPY-NUMBER SHUTTLE VECTORS [J].
CHRISTIANSON, TW ;
SIKORSKI, RS ;
DANTE, M ;
SHERO, JH ;
HIETER, P .
GENE, 1992, 110 (01) :119-122
[6]   CLONING AND CHARACTERIZATION OF GPD2, A 2ND GENE ENCODING SN-GLYCEROL 3-PHOSPHATE DEHYDROGENASE (NAD(+)) IN SACCHAROMYCES-CEREVISIAE, AND ITS COMPARISON WITH GPD1 [J].
ERIKSSON, P ;
ANDRE, L ;
ANSELL, R ;
BLOMBERG, A ;
ADLER, L .
MOLECULAR MICROBIOLOGY, 1995, 17 (01) :95-107
[7]   ISOLATION AND CHARACTERIZATION OF MUTANTS FROM SCHYZOSACCHAROMYCES-POMBE DEFECTIVE IN GLYCEROL CATABOLISM [J].
GANCEDO, C ;
LLOBELL, A ;
RIBAS, JC ;
LUCHI, F .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1986, 159 (01) :171-174
[8]  
GANSHEROFF LJ, 1995, GENETICS, V139, P523
[9]   PURIFICATION AND CHARACTERIZATION OF GLYCEROL KINASE FROM TRYPANOSOMA-BRUCEI [J].
KRAKOW, JL ;
WANG, CC .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 43 (01) :17-25
[10]   A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].
KYTE, J ;
DOOLITTLE, RF .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132